PKP3 interactions with MAPK-JNK-ERK1/2-mTOR pathway regulates autophagy and invasion in ovarian cancer.
Animals
Autophagy
Cell Movement
Cells, Cultured
Female
Humans
Immunohistochemistry
JNK Mitogen-Activated Protein Kinases
/ metabolism
Mice
Mice, Nude
Mitogen-Activated Protein Kinases
/ metabolism
Neoplasm Invasiveness
Neoplasms, Experimental
/ chemistry
Ovarian Neoplasms
/ chemistry
Plakophilins
/ analysis
TOR Serine-Threonine Kinases
/ metabolism
Autophagy
Mitogen-activated protein kinase
Ovarian cancer
Plakophilin
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
08 01 2019
08 01 2019
Historique:
received:
20
11
2018
accepted:
26
11
2018
pubmed:
12
12
2018
medline:
21
5
2019
entrez:
12
12
2018
Statut:
ppublish
Résumé
Armadillo-related proteins function in both signal transduction and cell adhesion, it also plays a central role in tumorigenesis. Plakophilin 3 (PKP3) is a member of the armadillo protein family. PKP3 has demonstrated a role in melanoma, breast cancer, gastric cancer, and other kind of cancers; however its role in ovarian cancer was not fully understood. In this study we explored the function and mechanisms of PKP3 in ovarian cancer. An elevated level of PKP3 was found in ovarian cancer tissues compared with normal tissues. PKP3 also modulate cellular proliferation and invasion in ovarian cancer. The ability of cellular proliferation, formation, and invasion was significantly decreased after the silencing of PKP3 in SKOV3 cells. While an over-expression of PKP3 in A2780 cells up-regulates the ability of cellular proliferation, formation, and invasion. As for the mechanism of PKP3, mTOR pathway was activated to regulate autophagy according to the interaction of PKP3 with the upstream of MAPK pathway. The result of this study support PKP3 as the oncogene candidate and a potential target for the treatment of ovarian cancer.
Identifiants
pubmed: 30527804
pii: S0006-291X(18)32598-1
doi: 10.1016/j.bbrc.2018.11.163
pii:
doi:
Substances chimiques
PKP3 protein, human
0
Plakophilins
0
MTOR protein, human
EC 2.7.1.1
TOR Serine-Threonine Kinases
EC 2.7.11.1
JNK Mitogen-Activated Protein Kinases
EC 2.7.11.24
Mitogen-Activated Protein Kinases
EC 2.7.11.24
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
646-653Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.