Different roles for aspartates and glutamates for cation permeation in bacterial sodium channels.
Amino Acid Sequence
Animals
Aspartic Acid
/ metabolism
Bacterial Proteins
/ chemistry
CHO Cells
Calcium
/ metabolism
Cations
Cell Membrane Permeability
Cricetinae
Cricetulus
Glutamic Acid
/ metabolism
Mutant Proteins
/ chemistry
Sodium
/ metabolism
Sodium Channels
/ chemistry
Structure-Activity Relationship
Time Factors
Water
/ chemistry
Ion channel selectivity
Molecular Dynamics
Voltage-gated sodium and calcium channels
Whole-cell patch clamp
Journal
Biochimica et biophysica acta. Biomembranes
ISSN: 1879-2642
Titre abrégé: Biochim Biophys Acta Biomembr
Pays: Netherlands
ID NLM: 101731713
Informations de publication
Date de publication:
01 02 2019
01 02 2019
Historique:
received:
19
09
2018
revised:
16
11
2018
accepted:
30
11
2018
pubmed:
12
12
2018
medline:
20
9
2019
entrez:
12
12
2018
Statut:
ppublish
Résumé
A key driving force for ion channel selectivity is represented by the negative charge of the Selectivity Filter carried by aspartate (D) and glutamate (E) residues. However, the structural effects and specific properties of D and E residues have not been extensively studied. In order to investigate this issue we studied the mutants of NaChBac channel with all possible combinations of D and E in the charged rings in position 191 and 192. Electrophysiological measurements showed significant Ca
Identifiants
pubmed: 30529079
pii: S0005-2736(18)30349-3
doi: 10.1016/j.bbamem.2018.11.011
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Cations
0
Mutant Proteins
0
NaChBac protein, bacteria
0
Sodium Channels
0
Water
059QF0KO0R
Aspartic Acid
30KYC7MIAI
Glutamic Acid
3KX376GY7L
Sodium
9NEZ333N27
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
495-503Informations de copyright
Copyright © 2018 The Author(s). Published by Elsevier B.V. All rights reserved.