Lowering line tension with high cholesterol content induces a transition from macroscopic to nanoscopic phase domains in model biomembranes.
Giant unilamellar vesicle
Line tension
Lipid domain
Liquid disordered phase
Liquid ordered phase
Journal
Biochimica et biophysica acta. Biomembranes
ISSN: 1879-2642
Titre abrégé: Biochim Biophys Acta Biomembr
Pays: Netherlands
ID NLM: 101731713
Informations de publication
Date de publication:
01 02 2019
01 02 2019
Historique:
received:
09
08
2018
revised:
26
11
2018
accepted:
27
11
2018
pubmed:
12
12
2018
medline:
20
9
2019
entrez:
12
12
2018
Statut:
ppublish
Résumé
Chemically simplified lipid mixtures are used here as models of the cell plasma membrane exoplasmic leaflet. In such models, phase separation and morphology transitions controlled by line tension in the liquid-disordered (Ld) + liquid-ordered (Lo) coexistence regime have been described [1]. Here, we study two four-component lipid mixtures at different cholesterol fractions: brain sphingomyelin (BSM) or 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC)/1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC)/cholesterol (Chol). On giant unilamellar vesicles (GUVs) display a nanoscopic-to-macroscopic transition of Ld + Lo phase domains as POPC is replaced by DOPC, and this transition also depends on the cholesterol fraction. Line tension decreases with increasing cholesterol mole fractions in both lipid mixtures. For the ternary BSM/DOPC/Chol mixture, the published phase diagram [19] requires a modification to show that when cholesterol mole fraction is >~0.33, coexisting phase domains become nanoscopic.
Identifiants
pubmed: 30529459
pii: S0005-2736(18)30348-1
doi: 10.1016/j.bbamem.2018.11.010
pmc: PMC6310626
mid: NIHMS1516371
pii:
doi:
Substances chimiques
Lipid Bilayers
0
Phosphatidylcholines
0
Sphingomyelins
0
Unilamellar Liposomes
0
Cholesterol
97C5T2UQ7J
1,2-oleoylphosphatidylcholine
EDS2L3ODLV
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
478-485Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM105684
Pays : United States
Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.
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