IL-33 Induces Murine Intestinal Goblet Cell Differentiation Indirectly via Innate Lymphoid Cell IL-13 Secretion.
Animals
Cell Differentiation
Coculture Techniques
Female
Goblet Cells
/ immunology
Immunity, Innate
Interleukin-13
/ immunology
Interleukin-33
/ immunology
Lymphocytes
/ immunology
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Mucin-2
/ genetics
Receptors, Cell Surface
/ genetics
Signal Transduction
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
15 01 2019
15 01 2019
Historique:
received:
27
02
2018
accepted:
06
11
2018
pubmed:
12
12
2018
medline:
16
10
2019
entrez:
12
12
2018
Statut:
ppublish
Résumé
Regulation of the intestinal mucus layer by goblet cells is important for preventing inflammation and controlling infection. IL-33, a cytokine upregulated in inflammatory bowel disease and helminth infection, induces intestinal goblet cells, but the mechanism remains unclear. Enteroids are three-dimensional structures of primary small intestinal epithelial cells that contain all differentiated intestinal epithelial cell types. We developed an enteroid-immune cell coculture model to determine the mechanism through which IL-33 affects intestinal goblet cell differentiation. We report that IL-33 does not directly induce goblet cell differentiation in murine enteroids; however, IL-13, a cytokine induced by IL-33, markedly induces goblet cells and gene expression consistent with goblet cell differentiation. When enteroids are cocultured with CD90
Identifiants
pubmed: 30530480
pii: jimmunol.1800292
doi: 10.4049/jimmunol.1800292
pmc: PMC6324976
mid: NIHMS1512156
doi:
Substances chimiques
Il4ra protein, mouse
0
Interleukin-13
0
Interleukin-33
0
Muc2 protein, mouse
0
Mucin-2
0
Receptors, Cell Surface
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
598-607Subventions
Organisme : NIDDK NIH HHS
ID : R03 DK110487
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK078392
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK117119
Pays : United States
Organisme : NIDDK NIH HHS
ID : K23 DK094832
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK116868
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK114123
Pays : United States
Informations de copyright
Copyright © 2019 by The American Association of Immunologists, Inc.
Références
Gastroenterology. 2017 May;152(6):1345-1357.e7
pubmed: 28132889
Clin Exp Allergy. 2011 Dec;41(12):1747-56
pubmed: 22092504
J Exp Med. 2000 Mar 20;191(6):1069-76
pubmed: 10727469
Nat Med. 2017 Aug;23(8):938-944
pubmed: 28714991
Gut. 2003 Oct;52(10):1442-7
pubmed: 12970137
Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11489-94
pubmed: 20534524
J Exp Med. 1989 Jan 1;169(1):59-72
pubmed: 2521244
Gastroenterology. 2001 May;120(6):1372-80
pubmed: 11313307
J Gastroenterol. 2010 Oct;45(10):999-1007
pubmed: 20405148
Cell Rep. 2016 May 24;15(8):1743-56
pubmed: 27184849
Proc Natl Acad Sci U S A. 2015 May 12;112(19):E2487-96
pubmed: 25918379
Nature. 2010 Apr 29;464(7293):1367-70
pubmed: 20200518
Int Immunol. 2008 Aug;20(8):1019-30
pubmed: 18550585
PLoS Pathog. 2010 May 13;6(5):e1000902
pubmed: 20485566
Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):282-7
pubmed: 23248269
Nature. 2016 Jan 14;529(7585):226-30
pubmed: 26762460
Nature. 2009 May 14;459(7244):262-5
pubmed: 19329995
Front Pediatr. 2017 Mar 03;5:40
pubmed: 28316967
J Cell Biol. 2011 Mar 7;192(5):767-80
pubmed: 21383077
Nature. 2015 Dec 24;528(7583):560-564
pubmed: 26649819
Gut. 2014 Feb;63(2):281-91
pubmed: 23426893
PLoS One. 2011;6(7):e22459
pubmed: 21829463
PLoS One. 2016 Aug 04;11(8):e0159609
pubmed: 27490946
Sci Rep. 2017 Mar 27;7:45270
pubmed: 28345602
Nat Immunol. 2011 Nov;12(11):1045-54
pubmed: 21946417
J Vis Exp. 2013 Oct 12;(80):
pubmed: 24145735
Inflamm Bowel Dis. 2015 Dec;21(12):2737-46
pubmed: 26313694
PLoS One. 2014 Jan 06;9(1):e84651
pubmed: 24400106
Infect Immun. 2013 Dec;81(12):4649-58
pubmed: 24101690
Sci Rep. 2017 Apr 12;7(1):851
pubmed: 28404987
Nature. 2016 Jan 14;529(7585):221-5
pubmed: 26675736
J Immunol. 2008 Feb 15;180(4):2443-9
pubmed: 18250453
J Immunol. 2014 Oct 15;193(8):4010-20
pubmed: 25217167
Curr Biol. 1998 Mar 12;8(6):339-42
pubmed: 9512421
Proc Natl Acad Sci U S A. 2015 Aug 25;112(34):10762-7
pubmed: 26243875
Proc Natl Acad Sci U S A. 2010 Apr 27;107(17):8017-22
pubmed: 20385815
PLoS One. 2011;6(11):e26898
pubmed: 22125602
Am J Physiol Gastrointest Liver Physiol. 2014 Apr 1;306(7):G547-56
pubmed: 24481605
Mucosal Immunol. 2008 Jul;1(4):252-64
pubmed: 19079187
Proc Natl Acad Sci U S A. 2018 Oct 2;115(40):E9362-E9370
pubmed: 30224451
J Exp Med. 2009 Dec 21;206(13):2947-57
pubmed: 19995957
Immunity. 2005 Nov;23(5):479-90
pubmed: 16286016
Proc Natl Acad Sci U S A. 1998 Jun 9;95(12):6930-5
pubmed: 9618516
Immunol Rev. 2014 Jul;260(1):8-20
pubmed: 24942678
PLoS Pathog. 2013;9(10):e1003698
pubmed: 24130494
Trends Immunol. 2016 May;37(5):321-333
pubmed: 27055914
Nature. 2014 Sep 25;513(7519):564-568
pubmed: 25043027