Methods to Probe Calcium Regulation by BCL-2 Family Members.


Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 12 12 2018
pubmed: 12 12 2018
medline: 1 6 2019
Statut: ppublish

Résumé

BCL-2 family members have additional roles beyond direct regulation of mitochondrial outer membrane permeabilization (MOMP) in apoptosis. One such important function is the release of calcium from the endoplasmic reticulum (ER), which critically contributes to the process of apoptosis. Here, we describe a protocol to measure calcium levels in the ER, mitochondria, and cytosol, with specific consideration of BCL-2 family biology.

Identifiants

pubmed: 30536006
doi: 10.1007/978-1-4939-8861-7_12
doi:

Substances chimiques

Proto-Oncogene Proteins c-bcl-2 0
Calcium SY7Q814VUP

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

173-183

Subventions

Organisme : NHLBI NIH HHS
ID : R01 HL131793
Pays : United States

Auteurs

Marcos A Carpio (MA)

Department of Pathology, Yale University School of Medicine, New Haven, CT, USA.
CIQUIBIC-Department of Biological Chemistry, National University of Cordoba, Cordoba, Argentina.

Samuel G Katz (SG)

Department of Pathology, Yale University School of Medicine, New Haven, CT, USA. Samuel.Katz@yale.edu.

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Classifications MeSH