50-Hz MF does not affect global DNA methylation of SH-SY5Y cells treated with the neurotoxin MPP
DNA methylation
ELF-MFs
MPP+
SH-SY5Y
epigenetics
Journal
Bioelectromagnetics
ISSN: 1521-186X
Titre abrégé: Bioelectromagnetics
Pays: United States
ID NLM: 8008281
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
07
05
2018
accepted:
23
10
2018
pubmed:
12
12
2018
medline:
21
5
2019
entrez:
12
12
2018
Statut:
ppublish
Résumé
Exposure to extremely low frequency magnetic fields (ELF-MFs) has been associated with an increased risk of neurodegenerative disorders. The underlying mechanisms, however, are still debated. Since epigenetics play a key role in the neurodegenerative process, we investigated whether exposure to ELF-MF (50 Hz, 1 mT) might affect global DNA methylation of SH-SY5Y dopaminergic-like neuroblastoma cells. We assessed the percentage of 5-methylcytosine (5-mC) of three repetitive interspersed sequences (ALU, LINE-1, or SATα), through pyrosequencing analysis. We demonstrated that ELF exposure (up to 72 h) does not induce any change in the methylation pattern of ALU, LINE-1, and SATα in both proliferating and differentiated SH-SY5Y cells. Furthermore, when administered in combination with 1-methyl-4-phenylpyridinium (MPP
Substances chimiques
Neurotoxins
0
1-Methyl-4-phenylpyridinium
R865A5OY8J
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
33-41Informations de copyright
© 2018 Bioelectromagnetics Society.