Combinatorial Utilization of Murine Embryonic Stem Cells and In Vivo Models to Study Human Congenital Heart Disease.

Animals Disease Models, Animal Embryonic Stem Cells / cytology Gene Expression Regulation, Developmental Heart / growth & development Heart Defects, Congenital / genetics Homeobox Protein Nkx-2.5 / genetics Humans Mice Myocytes, Cardiac / cytology RNA / isolation & purification
cardiac differentiation embryoid bodies embryos hanging drops mouse embryonic stem cells

Journal

Current protocols in stem cell biology
ISSN: 1938-8969
Titre abrégé: Curr Protoc Stem Cell Biol
Pays: United States
ID NLM: 101470226

Informations de publication

Date de publication:
02 2019
Historique:
pubmed: 15 12 2018
medline: 12 7 2019
entrez: 15 12 2018
Statut: ppublish

Résumé

We have established an in vitro model of the human congenital heart defect (CHD)-associated mutation NKX2.5 R141C. We describe the use of the hanging drop method to differentiate Nkx2.5

Identifiants

DOI: 10.1002/cpsc.75 PMID: 30548532
pubmed: 30548532
doi: 10.1002/cpsc.75
doi:

Substances chimiques

Homeobox Protein Nkx-2.5 0
Nkx2-5 protein, mouse 0
RNA 63231-63-0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e75

Subventions

Organisme : CIHR
ID : MOP 53277
Pays : Canada
Organisme : CIHR
ID : MOP 119458
Pays : Canada
Organisme : CIHR
ID : MOP 119470
Pays : Canada

Informations de copyright

© 2018 John Wiley & Sons, Inc.

Auteurs

Abeer Zakariyah (A)

Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario, Canada.

Rashida Rajgara (R)

Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario, Canada.
Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada.

Michael Shelton (M)

Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario, Canada.

Alexandre Blais (A)

Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario, Canada.
Ottawa Institute of Systems Biology, Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada.
Center for Neuromuscular Disease, University of Ottawa, Ottawa, Ontario, Canada.

Ilona S Skerjanc (IS)

Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario, Canada.

Patrick G Burgon (PG)

Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada.
Center for Neuromuscular Disease, University of Ottawa, Ottawa, Ontario, Canada.
Department of Medicine, Division of Cardiology, University of Ottawa, Ottawa, Ontario, Canada.
University of Ottawa Heart Institute, Ottawa, Ontario, Canada.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Combinatorial Utilization of Murine Embryonic...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Combinatorial Utilization of Murine Embryonic...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches embryonnaires › Combinatorial Utilization of Murine Embryonic...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes au cours du développement › Combinatorial Utilization of Murine Embryonic...
questionsmedicales.fr › Système cardiovasculaire › Coeur › Combinatorial Utilization of Murine Embryonic...
questionsmedicales.fr › Malformations et maladies congénitales, héréditaires et néonatales › Malformations › Malformations cardiovasculaires › Cardiopathies congénitales › Combinatorial Utilization of Murine Embryonic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Protéine homéotique Nkx-2.5 › Combinatorial Utilization of Murine Embryonic...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Combinatorial Utilization of Murine Embryonic...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Combinatorial Utilization of Murine Embryonic...
questionsmedicales.fr › Cellules › Cellules musculaires › Myocytes cardiaques › Combinatorial Utilization of Murine Embryonic...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › Combinatorial Utilization of Murine Embryonic...