Risk of Chlamydia abortus transmission via embryo transfer using in vitro produced early bovine embryos.
Bovine embryo
Chlamydia abortus
Embryo transfer
IETS
PCR
Zona pellucida
Journal
Theriogenology
ISSN: 1879-3231
Titre abrégé: Theriogenology
Pays: United States
ID NLM: 0421510
Informations de publication
Date de publication:
01 Mar 2019
01 Mar 2019
Historique:
received:
05
06
2018
revised:
27
11
2018
accepted:
30
11
2018
pubmed:
15
12
2018
medline:
12
2
2019
entrez:
15
12
2018
Statut:
ppublish
Résumé
The objectives of this study were to determine (i) whether Chlamydia (C.) abortus would adhere to the intact zona pellucida (ZP-intact) of early in vitro produced bovine embryos; (ii) whether the bacteria would adhere to the embryos (ZP-free) after in vitro infection; and (iii) the efficacy of the International Embryo Transfer Society (IETS) washing protocol. The experimentation was made twice. For each replicate 100 (8-16-cell) bovine embryos produced in vitro were randomly divided into 10 batches. Height batches (4 ZP-intact and 4 ZP-free) of 10 embryos were incubated in a medium containing 4 × 10
Identifiants
pubmed: 30551017
pii: S0093-691X(18)30323-6
doi: 10.1016/j.theriogenology.2018.11.033
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
114-120Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.