Hydroxytyrosol supplementation ameliorates the metabolic disturbances in white adipose tissue from mice fed a high-fat diet through recovery of transcription factors Nrf2, SREBP-1c, PPAR-γ and NF-κB.
Adipose Tissue, White
/ drug effects
Animals
Antioxidants
/ pharmacology
Diet, High-Fat
/ adverse effects
Male
Metabolic Diseases
/ drug therapy
Mice
Mice, Inbred C57BL
NF-E2-Related Factor 2
/ metabolism
NF-kappa B
/ antagonists & inhibitors
PPAR gamma
/ metabolism
Phenylethyl Alcohol
/ analogs & derivatives
Sterol Regulatory Element Binding Protein 1
/ antagonists & inhibitors
Transcription Factors
/ metabolism
Hydroxytyrosol
NF-κB
Nrf2
Oxidative stress
PPAR-γ
White adipose tissue
Journal
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
ISSN: 1950-6007
Titre abrégé: Biomed Pharmacother
Pays: France
ID NLM: 8213295
Informations de publication
Date de publication:
Jan 2019
Jan 2019
Historique:
received:
13
09
2018
revised:
19
11
2018
accepted:
25
11
2018
entrez:
16
12
2018
pubmed:
16
12
2018
medline:
2
4
2019
Statut:
ppublish
Résumé
White adipose tissue (WAT) have a relevant metabolic and inflammatory function, in overweight or obesity conditions. In this regard, the WAT under over feeding nutrition present a significant increment in oxidative stress, pro-inflammatory status and depletion of n-3 long chain polyunsaturated fatty acid. Hydroxytyrosol (HT) is a polyphenol with important cytoprotective effects, and this molecule can modulate the gene expression, transcription factors and enzymatic activity. Therefore, the purpose of this study was evaluate the anti-inflammatory, anti-oxidant and anti-lipogenic effects of HT supplementation mice and the molecular adaptations involved, on dysfunctional WAT from high-fat diet (HFD)-fed mice. Male C57BL/6 J mice received (i) control diet (10% fat); (ii) control diet + HT (daily doses of 5 mg kg body weight), (iii) HFD (60% fat); or (iv) HFD + HT for 12 weeks. HFD-fed mice exhibited: (i) WAT hypertrophy; (ii) oxidative stress and depletion of antioxidant defenses, (iii) increased lipogenesis and pro-inflammatory status, (iv) depletion of n-3 LCPUFA and (v) up-regulation of NF-κB and SREBP 1c with down-regulation Nrf2, and PPAR-γ. HT supplementation attenuated the metabolic impairment produced by HFD in WAT, attenuating increment of NF-κB and SREBP 1c, and increasing the activity of Nrf2 and PPAR-γ. Supplementation with HT improve the WAT dysfunction induced by HDF in mice through the modulation of transcription factors NF-κB, Nrf2, SREBP-1c and PPAR-γ as well as their target genes, involved in inflammation, antioxidant defenses and lipogenesis.
Sections du résumé
BACKGROUND
BACKGROUND
White adipose tissue (WAT) have a relevant metabolic and inflammatory function, in overweight or obesity conditions. In this regard, the WAT under over feeding nutrition present a significant increment in oxidative stress, pro-inflammatory status and depletion of n-3 long chain polyunsaturated fatty acid. Hydroxytyrosol (HT) is a polyphenol with important cytoprotective effects, and this molecule can modulate the gene expression, transcription factors and enzymatic activity.
OBJECTIVE
OBJECTIVE
Therefore, the purpose of this study was evaluate the anti-inflammatory, anti-oxidant and anti-lipogenic effects of HT supplementation mice and the molecular adaptations involved, on dysfunctional WAT from high-fat diet (HFD)-fed mice.
METHODS AND RESULTS
RESULTS
Male C57BL/6 J mice received (i) control diet (10% fat); (ii) control diet + HT (daily doses of 5 mg kg body weight), (iii) HFD (60% fat); or (iv) HFD + HT for 12 weeks. HFD-fed mice exhibited: (i) WAT hypertrophy; (ii) oxidative stress and depletion of antioxidant defenses, (iii) increased lipogenesis and pro-inflammatory status, (iv) depletion of n-3 LCPUFA and (v) up-regulation of NF-κB and SREBP 1c with down-regulation Nrf2, and PPAR-γ. HT supplementation attenuated the metabolic impairment produced by HFD in WAT, attenuating increment of NF-κB and SREBP 1c, and increasing the activity of Nrf2 and PPAR-γ.
CONCLUSION
CONCLUSIONS
Supplementation with HT improve the WAT dysfunction induced by HDF in mice through the modulation of transcription factors NF-κB, Nrf2, SREBP-1c and PPAR-γ as well as their target genes, involved in inflammation, antioxidant defenses and lipogenesis.
Identifiants
pubmed: 30551508
pii: S0753-3322(18)36561-2
doi: 10.1016/j.biopha.2018.11.120
pii:
doi:
Substances chimiques
Antioxidants
0
NF-E2-Related Factor 2
0
NF-kappa B
0
Nfe2l2 protein, mouse
0
PPAR gamma
0
Srebf1 protein, mouse
0
Sterol Regulatory Element Binding Protein 1
0
Transcription Factors
0
3,4-dihydroxyphenylethanol
10597-60-1
Phenylethyl Alcohol
ML9LGA7468
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2472-2481Informations de copyright
Copyright © 2018 The Authors. Published by Elsevier Masson SAS.. All rights reserved.