Silencing of lncRNA LINC00514 inhibits the malignant behaviors of papillary thyroid cancer through miR-204-3p/CDC23 axis.
Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome
/ genetics
Base Sequence
Cell Line, Tumor
Disease Progression
Gene Expression Regulation, Neoplastic
Gene Silencing
Humans
MicroRNAs
/ genetics
RNA, Long Noncoding
/ genetics
Signal Transduction
/ genetics
Thyroid Cancer, Papillary
/ genetics
Up-Regulation
/ genetics
Invasion
LINC00514
LncRNA
PTC
Proliferation
Journal
Biochemical and biophysical research communications
ISSN: 1090-2104
Titre abrégé: Biochem Biophys Res Commun
Pays: United States
ID NLM: 0372516
Informations de publication
Date de publication:
22 01 2019
22 01 2019
Historique:
received:
26
11
2018
accepted:
06
12
2018
pubmed:
17
12
2018
medline:
11
9
2019
entrez:
17
12
2018
Statut:
ppublish
Résumé
Numerous studies have provided that long noncoding RNAs (lncRNAs) possess important roles in regulating tumorigenesis. However, up to data, the role of LINC00514 in cancer, including thyroid cancer, remains unknown. In the present study, we found that LINC00514 expression was significantly upregulated in papillary thyroid cancer (PTC) tissues by bioinformatics analysis. Loss-of-function studies revealed that LINC00514 silencing inhibited the proliferation, migration and invasion of PTC cells while promoting apoptosis in vitro. Moreover, LINC00514 knockdown suppressed PTC growth in vivo. RNA-FISH showed that LINC00514 mainly locates in the nucleus of PTC cells. Through bioinformatics prediction, we identified that LINC00514 served as the sponge for miR-204-3p, and miR-204-3p directly targeted CDC23. Thus, LINC00514 promoted CDC23 expression via restraining miR-204-3p activity, leading to PTC progression. In sum, our findings demonstrated that LINC00514 contributes to PTC progression and might be a potential target for PTC therapy.
Identifiants
pubmed: 30553447
pii: S0006-291X(18)32694-9
doi: 10.1016/j.bbrc.2018.12.051
pii:
doi:
Substances chimiques
Apc8 Subunit, Anaphase-Promoting Complex-Cyclosome
0
CDC23 protein, human
0
MIRN204 microRNA, human
0
MicroRNAs
0
RNA, Long Noncoding
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1145-1148Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.