Hybridization induced fluorescence enhanced DNA-Ag nanocluster/aptamer probe for detection of prostate-specific antigen.

Aptamers, Nucleotide / chemistry Biosensing Techniques / methods DNA Probes / chemistry Fluorescence Humans Limit of Detection Metal Nanoparticles / chemistry Nucleic Acid Hybridization / methods Prostate-Specific Antigen / blood Silver / chemistry Spectrometry, Fluorescence
Ag nanocluster Aptamer DNA-AgNC fluorescent probe Prostate-specific antigen

Journal

Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Titre abrégé: Colloids Surf B Biointerfaces
Pays: Netherlands
ID NLM: 9315133

Informations de publication

Date de publication:
01 Mar 2019
Historique:
received: 08 10 2018
revised: 06 12 2018
accepted: 06 12 2018
pubmed: 17 12 2018
medline: 14 6 2019
entrez: 17 12 2018
Statut: ppublish

Résumé

In this work, a label-free Ag nanocluster (AgNC)-based fluorescent probe is proposed to detect tumor marker, prostate-specific antigen (PSA). In the experiments, DNA sequences containing segments complemented to different parts of PSA aptamer were used to synthesize DNA-Ag nanoclusters (DNA-AgNC). Some of the obtained specific DNA-AgNC exhibited significant fluorescence increase after hybridization with PSA aptamer. Based on this, a simple DNA-AgNC/aptamer hybridization probe was fabricated for PSA detection using fluorescence quenching, because competitively specific binding between PSA and its aptamer inhibited the fluorescence enhancement effect of PSA aptamer on DNA-AgNC. The sequence of template DNA, pH and salt concentration of binding buffer, and the concentration of aptamer were optimized. Under optimum conditions, the concentration of PSA within the range of 2-150 ng mL

Identifiants

DOI: 10.1016/j.colsurfb.2018.12.013 PMID: 30554014
pubmed: 30554014
pii: S0927-7765(18)30892-0
doi: 10.1016/j.colsurfb.2018.12.013
pii:
doi:

Substances chimiques

Aptamers, Nucleotide 0
DNA Probes 0
Silver 3M4G523W1G
Prostate-Specific Antigen EC 3.4.21.77

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

358-364

Informations de copyright

Copyright © 2018 Elsevier B.V. All rights reserved.

Auteurs

Bi-Yun Fang (BY)

Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, Hubei, PR China; Key Laboratory of Biomedical Photonics (HUST), Ministry of Education, Huazhong University of Science and Technology, Wuhan, 430074, Hubei, PR China; Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Health Science Center, Xi'an Jiaotong University, Xi'an, Shanxi, 710061, PR China.

Jie An (J)

Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, Hubei, PR China.

Bo Liu (B)

Key Laboratory of Biomedical Photonics (HUST), Ministry of Education, Huazhong University of Science and Technology, Wuhan, 430074, Hubei, PR China.

Yuan-Di Zhao (YD)

Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, Hubei, PR China; Key Laboratory of Biomedical Photonics (HUST), Ministry of Education, Huazhong University of Science and Technology, Wuhan, 430074, Hubei, PR China. Electronic address: zydi@mail.hust.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Aptamères nucléotidiques › Hybridization induced fluorescence enhanced...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › Hybridization induced fluorescence enhanced...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Sondes moléculaires › Sondes d'acide nucléique › Sondes d'ADN › Hybridization induced fluorescence enhanced...
questionsmedicales.fr › Phénomènes physiques › Phénomènes optiques › Fluorescence › Hybridization induced fluorescence enhanced...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Hybridization induced fluorescence enhanced...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Limite de détection › Hybridization induced fluorescence enhanced...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanoparticules › Nanoparticules métalliques › Hybridization induced fluorescence enhanced...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Hybridation d'acides nucléiques › Hybridization induced fluorescence enhanced...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Marqueurs biologiques tumoraux › Antigène spécifique de la prostate › Hybridization induced fluorescence enhanced...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux lourds › Argent › Hybridization induced fluorescence enhanced...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectrométrie de fluorescence › Hybridization induced fluorescence enhanced...