Is it possible to combine photodynamic therapy and application of dinitrosyl iron complexes in the wound treatment?

We have studied the effect of interactions between dinitrosyl iron complexes with thiol-containing ligands (DNIC-TL) and diglucamine salt of chlorine e6 (photoditazine, PD) on the rate of photosensitized oxidation of a model organic substrate - tryptophan - in the presence and absence of an amphiphilic polymer, Pluronic F127, as well as on the DNIC-TL and PD photostability. Using EPR and UV spectroscopy, we determined the rate constants for photodegradation of mono- and dinuclear DNIC-TL and PD, respectively. The presence of the photosensitizer and Pluronic F127 has been shown to have a negligible effect on the rate of photodestruction of mono- and dinuclear DNIC-TL, taking into account the changing DNIC-TL and PD concentrations in the photoexcitation conditions. At the same time, in the DNIC-TL presence, the rate of PD photodestruction increases, however, addition of Pluronic F127 leads to a decrease in the rate constant of PD photodestruction. The latter circumstance creates an opportunity for a simultaneous application of DNIC-TL and photodynamic therapy in the wound treatment without losing the PDT efficiency. Indeed, photodynamic therapy in combination with DNIC-TL facilitated skin wound healing in laboratory rats. As shown by a morphological study, application of the DNIC-TL-PD-F127 complex with the subsequent photoactivation was beneficial in reducing inflammation and stimulating regenerative processes.

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