Imaging cellular ultrastructures using expansion microscopy (U-ExM).
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
30
04
2018
accepted:
31
10
2018
pubmed:
19
12
2018
medline:
25
6
2019
entrez:
19
12
2018
Statut:
ppublish
Résumé
Determining the structure and composition of macromolecular assemblies is a major challenge in biology. Here we describe ultrastructure expansion microscopy (U-ExM), an extension of expansion microscopy that allows the visualization of preserved ultrastructures by optical microscopy. This method allows for near-native expansion of diverse structures in vitro and in cells; when combined with super-resolution microscopy, it unveiled details of ultrastructural organization, such as centriolar chirality, that could otherwise be observed only by electron microscopy.
Identifiants
pubmed: 30559430
doi: 10.1038/s41592-018-0238-1
pii: 10.1038/s41592-018-0238-1
pmc: PMC6314451
mid: EMS80371
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
71-74Références
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