Cold Ischemia Score: An mRNA Assay for the Detection of Extended Cold Ischemia in Formalin-Fixed, Paraffin-Embedded Tissue.
Cold Ischemia
/ methods
Colonic Neoplasms
/ genetics
Female
Fixatives
/ chemistry
Formaldehyde
/ chemistry
Humans
Kidney Neoplasms
/ genetics
Male
Neoplasm Proteins
/ genetics
Ovarian Neoplasms
/ genetics
Paraffin Embedding
/ methods
RNA, Messenger
/ genetics
Reverse Transcriptase Polymerase Chain Reaction
/ methods
Time Factors
Tissue Fixation
/ methods
Transcriptome
None
cancer
degradation
stability
transcript
Journal
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
ISSN: 1551-5044
Titre abrégé: J Histochem Cytochem
Pays: United States
ID NLM: 9815334
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
pubmed:
19
12
2018
medline:
28
12
2019
entrez:
19
12
2018
Statut:
ppublish
Résumé
Although there are thousands of formalin-fixed paraffin-embedded (FFPE) tissue blocks potentially available for scientific research, many are of questionable quality, partly due to unknown preanalytical variables. We analyzed FFPE tissue biospecimens as part of the National Cancer Institute (NCI) Biospecimen Preanalytical Variables program to identify mRNA markers denoting cold ischemic time. The mRNA was extracted from colon, kidney, and ovary cancer FFPE blocks (40 patients, 10-12 hr fixation time) with 1, 2, 3, and 12 hr cold ischemic times, then analyzed using qRT-PCR for 23 genes selected following a literature search. No genes tested could determine short ischemic times (1-3 hr). However, a combination of three unstable genes normalized to a more stable gene could generate a "Cold Ischemia Score" that could distinguish 1 to 3 hr cold ischemia from 12 hr cold ischemia with 62% sensitivity and 84% specificity.
Identifiants
pubmed: 30562131
doi: 10.1369/0022155418819967
pmc: PMC6393842
doi:
Substances chimiques
Fixatives
0
Neoplasm Proteins
0
RNA, Messenger
0
Formaldehyde
1HG84L3525
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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