Phenotypic and molecular characterization of Acinetobacter baumannii isolates causing lower respiratory infections among ICU patients.
Acinetobacter Infections
/ epidemiology
Acinetobacter baumannii
/ drug effects
Anti-Bacterial Agents
/ pharmacology
Bacterial Proteins
/ genetics
Biofilms
/ drug effects
Carbapenem-Resistant Enterobacteriaceae
/ genetics
Carbapenems
/ pharmacology
Colistin
/ pharmacology
Cross Infection
Drug Resistance, Multiple, Bacterial
/ genetics
Gene Transfer, Horizontal
Genes, Bacterial
/ genetics
Genotype
Hospitals
Humans
India
Intensive Care Units
Microbial Sensitivity Tests
Microbiological Techniques
Molecular Typing
Phenotype
Prevalence
Prospective Studies
Respiratory Tract Infections
/ epidemiology
Species Specificity
Virulence
beta-Lactamases
/ genetics
Carbapenem resistant Acinetobacter baumannii (CRAB)
Lower respiratory tract infections (LRTI)
Multidrug resistant
Pulse field gel electrophoresis (PFGE)
Journal
Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
22
08
2018
revised:
16
11
2018
accepted:
11
12
2018
pubmed:
19
12
2018
medline:
20
3
2019
entrez:
19
12
2018
Statut:
ppublish
Résumé
Multi-drug resistant Acinetobacter baumannii has emerged as important nosocomial pathogen associated with various infections including lower respiratory tract. Limited therapeutic options contribute to increased morbidity and mortality. Acinetobacter baumannii has the ability to persist in the environment for prolonged periods. Breach in infection control practices increases the chances of cross transmission between patients and inter/intraspecies transmission of resistance elements. The present prospective work was conducted among patients with lower respiratory tract infections (LRTI) in the intensive care unit (ICU) to study the etiology with special reference to Acinetobacter baumannii and the role of immediate patient environment in the ICU as possible source of infection. Acinetobacter baumannii were characterized for antimicrobial susceptibility, mechanism of carbapenem resistance and virulence determinants. Molecular typing of the clinical and environmental isolates was undertaken to study the probable modes of transmission. Appropriate respiratory samples from 107 patients with LRTI admitted to ICU during September 2016 to March 2017 were studied for likely bacterial pathogens. Environmental samples (n = 71) were also screened. All the samples were processed using conventional microbiological methods. Consecutive Acinetobacter spp. isolated from clinical and environmental (health care workers and environment from ICU) samples were included in the study. Antimicrobial susceptibility was performed as per CLSI guidelines. Carbapenem resistance, mediated by carbapenemase genes (bla The prevalence of Acinetobacter baumannii was 26.2% (28/107) and 11.26% (8/71) among patients with LRTI and environmental samples respectively. The carbapenem resistance was high, 96.42% (27/28) and 87.5% (7/8) in clinical and environmental isolates respectively. The most common carbapenemase associated with resistance was bla Carbapenem resistant Acinetobacter baumannii (CRAB) is an important cause of LRTI in the ICU. PFGE suggests spread of carbapenem resistant isolates via cross transmission among patients and the environment. The detection of bla
Sections du résumé
BACKGROUND
BACKGROUND
Multi-drug resistant Acinetobacter baumannii has emerged as important nosocomial pathogen associated with various infections including lower respiratory tract. Limited therapeutic options contribute to increased morbidity and mortality. Acinetobacter baumannii has the ability to persist in the environment for prolonged periods. Breach in infection control practices increases the chances of cross transmission between patients and inter/intraspecies transmission of resistance elements. The present prospective work was conducted among patients with lower respiratory tract infections (LRTI) in the intensive care unit (ICU) to study the etiology with special reference to Acinetobacter baumannii and the role of immediate patient environment in the ICU as possible source of infection. Acinetobacter baumannii were characterized for antimicrobial susceptibility, mechanism of carbapenem resistance and virulence determinants. Molecular typing of the clinical and environmental isolates was undertaken to study the probable modes of transmission.
MATERIALS AND METHODS
METHODS
Appropriate respiratory samples from 107 patients with LRTI admitted to ICU during September 2016 to March 2017 were studied for likely bacterial pathogens. Environmental samples (n = 71) were also screened. All the samples were processed using conventional microbiological methods. Consecutive Acinetobacter spp. isolated from clinical and environmental (health care workers and environment from ICU) samples were included in the study. Antimicrobial susceptibility was performed as per CLSI guidelines. Carbapenem resistance, mediated by carbapenemase genes (bla
RESULTS
RESULTS
The prevalence of Acinetobacter baumannii was 26.2% (28/107) and 11.26% (8/71) among patients with LRTI and environmental samples respectively. The carbapenem resistance was high, 96.42% (27/28) and 87.5% (7/8) in clinical and environmental isolates respectively. The most common carbapenemase associated with resistance was bla
DISCUSSION
CONCLUSIONS
Carbapenem resistant Acinetobacter baumannii (CRAB) is an important cause of LRTI in the ICU. PFGE suggests spread of carbapenem resistant isolates via cross transmission among patients and the environment. The detection of bla
Identifiants
pubmed: 30562602
pii: S0882-4010(18)31466-9
doi: 10.1016/j.micpath.2018.12.023
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
Carbapenems
0
beta-Lactamases
EC 3.5.2.6
beta-lactamase NDM-1
EC 3.5.2.6
beta-lactamase OXA-23, Acinetobacter baumannii
EC 3.5.2.6
beta-lactamase OXA-24
EC 3.5.2.6
beta-lactamase OXA-58, Acinetobacter baumannii
EC 3.5.2.6
carbapenemase
EC 3.5.2.6
Colistin
Z67X93HJG1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
75-81Informations de copyright
Copyright © 2018. Published by Elsevier Ltd.