Tissue microenvironment initiates an immune response to structural components of Staphylococcus aureus.
Cell Communication
Culture Media, Conditioned
/ pharmacology
Cytokines
/ immunology
Dendritic Cells
/ immunology
Fibroblasts
/ immunology
Humans
Immune System
Inflammation
Keratinocytes
/ immunology
Langerhans Cells
/ immunology
Monocytes
/ immunology
Phenotype
Skin
/ immunology
Staphylococcal Infections
/ immunology
Staphylococcus aureus
Up-Regulation
Staphylococcus aureus
dendritic cells
inflammation
microenvironment
structural components
Journal
Experimental dermatology
ISSN: 1600-0625
Titre abrégé: Exp Dermatol
Pays: Denmark
ID NLM: 9301549
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
18
07
2018
revised:
13
11
2018
accepted:
13
12
2018
pubmed:
20
12
2018
medline:
18
6
2020
entrez:
20
12
2018
Statut:
ppublish
Résumé
Cell-to-cell communication in skin participates to the maintenance of homeostatic responses to foreign substances. Certain strains of Staphylococcus (S) aureus are vicious pathogens that cause deleterious effects in host cells and tissues. Both secreted toxins and structural components of S. aureus trigger an immune response, though how S. aureus stimulates host immune responses is poorly understood. We explored here how keratinocytes and fibroblasts initiate the first steps of an immune response by activating dendritic cells (DCs) through recognition of structural components of S. aureus. We treated monocyte-derived Langerhans cells (moLCs) and monocyte-derived DCs (moDCs) with conditioned media from keratinocytes (K-CM) and fibroblasts (F-CM) treated with heat-killed S. aureus (HKSA) respectively, or directly with HKSA. Immune and inflammatory responses from keratinocytes, fibroblasts, moLCs and moDCs were assessed by analysis of cell surface markers and cytokine production using flow cytometry, real-time PCR and ELISA assays. K-CM and F-CM increased the expression of CD86 and HLA-DR on moLCs and moDCs, in association with a specific cytokine profile. K-CM upregulated TNFA, IL-1B and GM-CSF mRNA expression in moLCs, while F-CM upregulated IL-12 and downregulated TNFA and TGFB mRNA expression in moDCs. Additionally, F-CM attenuated the induction of an inflammatory profile in monocytes. The recognition of structural components from S. aureus by cutaneous microenvironment induces the activation and the expression of specific cytokines from LCs and DCs.
Identifiants
pubmed: 30566255
doi: 10.1111/exd.13864
pmc: PMC6706075
mid: NIHMS1045460
doi:
Substances chimiques
Culture Media, Conditioned
0
Cytokines
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
161-168Subventions
Organisme : SILAB
Pays : International
Organisme : NIAMS NIH HHS
ID : R01 AR062025
Pays : United States
Informations de copyright
© 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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