EPR Spectroscopy Detects Various Active State Conformations of the Transcriptional Regulator CueR.
DEER
EPR spectroscopy
copper regulators
protein-DNA
spin labeling
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
04 03 2019
04 03 2019
Historique:
received:
16
09
2018
revised:
29
11
2018
pubmed:
20
12
2018
medline:
28
8
2020
entrez:
20
12
2018
Statut:
ppublish
Résumé
The interactions between proteins and their specific DNA sequences are the basis of many cellular processes. Hence, developing methods to build an atomic level picture of these interactions helps improve our understanding of key cellular mechanisms. CueR is an Escherichia coli copper-sensing transcription regulator. The inhibition of copper-sensing transcription regulators can kill pathogens, without harming the host. Several spectroscopic studies and crystallographic data have suggested that changes in the conformation of both the DNA and the protein control transcription. However, due to the inadequate resolution of these methods, the exact number of active conformations of CueR has not been determined. Resolving the structure of CueR in its active state is highly important for the development of specific inhibitors. Herein, the potential of double-histidine (dHis)-based Cu
Identifiants
pubmed: 30566257
doi: 10.1002/anie.201810656
doi:
Substances chimiques
DNA, Bacterial
0
Escherichia coli Proteins
0
Trans-Activators
0
cueR protein, E coli
0
Copper
789U1901C5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3053-3056Subventions
Organisme : Israel Science Foundation
ID : 176/16
Pays : International
Organisme : National Science Foundation (Division of Molecular and Cellular Biosciences)
ID : 1613007
Pays : International
Organisme : Directorate for Biological Sciences
ID : 1613007
Pays : International
Informations de copyright
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.