The Tudor protein Veneno assembles the ping-pong amplification complex that produces viral piRNAs in Aedes mosquitoes.
Aedes
/ genetics
Animals
Argonaute Proteins
/ genetics
DNA Transposable Elements
/ genetics
Drosophila Proteins
/ genetics
Drosophila melanogaster
/ genetics
Germ Cells
/ growth & development
Mosquito Vectors
/ genetics
Multiprotein Complexes
/ genetics
RNA, Small Interfering
/ genetics
Tudor Domain
/ genetics
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
18 03 2019
18 03 2019
Historique:
accepted:
10
12
2018
revised:
28
11
2018
received:
24
10
2018
pubmed:
20
12
2018
medline:
16
10
2019
entrez:
20
12
2018
Statut:
ppublish
Résumé
PIWI-interacting RNAs (piRNAs) comprise a class of small RNAs best known for suppressing transposable elements in germline tissues. The vector mosquito Aedes aegypti encodes seven PIWI genes, four of which are somatically expressed. This somatic piRNA pathway generates piRNAs from viral RNA during infection with cytoplasmic RNA viruses through ping-pong amplification by the PIWI proteins Ago3 and Piwi5. Yet, additional insights into the molecular mechanisms mediating non-canonical piRNA production are lacking. TUDOR-domain containing (Tudor) proteins facilitate piRNA biogenesis in Drosophila melanogaster and other model organisms. We thus hypothesized that Tudor proteins are required for viral piRNA production and performed a knockdown screen targeting all A. aegypti Tudor genes. Knockdown of the Tudor genes AAEL012437, Vreteno, Yb, SMN and AAEL008101-RB resulted in significantly reduced viral piRNA levels, with AAEL012437-depletion having the strongest effect. This protein, which we named Veneno, associates directly with Ago3 in an sDMA-dependent manner and localizes in cytoplasmic foci reminiscent of piRNA processing granules of Drosophila. Veneno-interactome analyses reveal a network of co-factors including the orthologs of the Drosophila piRNA pathway components Vasa and Yb, which in turn interacts with Piwi5. We propose that Veneno assembles a multi-protein complex for ping-pong dependent piRNA production from viral RNA.
Identifiants
pubmed: 30566680
pii: 5253060
doi: 10.1093/nar/gky1266
pmc: PMC6411831
doi:
Substances chimiques
AGO3 protein, Drosophila
0
Argonaute Proteins
0
DNA Transposable Elements
0
Drosophila Proteins
0
Multiprotein Complexes
0
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2546-2559Informations de copyright
© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.
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