Expansion microscopy: principles and uses in biological research.
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
15
02
2018
accepted:
10
10
2018
entrez:
22
12
2018
pubmed:
24
12
2018
medline:
25
6
2019
Statut:
ppublish
Résumé
Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial resolution. We recently discovered that preserved biological specimens can be physically expanded in an isotropic fashion through a chemical process. Expansion microscopy (ExM) allows nanoscale imaging of biological specimens with conventional microscopes, decrowds biomolecules in support of signal amplification and multiplexed readout chemistries, and makes specimens transparent. We review the principles of how ExM works, advances in the technology made by our group and others, and its applications throughout biology and medicine.
Identifiants
pubmed: 30573813
doi: 10.1038/s41592-018-0219-4
pii: 10.1038/s41592-018-0219-4
pmc: PMC6373868
mid: NIHMS1010719
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
33-41Subventions
Organisme : NINDS NIH HHS
ID : R01 NS102727
Pays : United States
Organisme : NHGRI NIH HHS
ID : RM1 HG008525
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB024261
Pays : United States
Organisme : NIMH NIH HHS
ID : R41 MH112318
Pays : United States
Organisme : NINDS NIH HHS
ID : DP1 NS087724
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH110932
Pays : United States
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