Novel circular RNA circNF1 acts as a molecular sponge, promoting gastric cancer by absorbing miR-16.
circNF1
gastric carcinoma
miR-16
proliferation
Journal
Endocrine-related cancer
ISSN: 1479-6821
Titre abrégé: Endocr Relat Cancer
Pays: England
ID NLM: 9436481
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
14
12
2018
accepted:
14
12
2018
pubmed:
24
12
2018
medline:
17
7
2020
entrez:
22
12
2018
Statut:
ppublish
Résumé
Circular RNAs (circRNAs) are a new class of RNA involved in multiple human malignancies. However, limited information exists regarding the involvement of circRNAs in gastric carcinoma (GC). Therefore, we sought to identify novel circRNAs, their functions and mechanisms in gastric carcinogenesis. We analyzed next-generation RNA sequencing data from GC tissues and cell lines, identifying 75,201 candidate circRNAs. Among these, we focused on one novel circRNA, circNF1 , which was upregulated in GC tissues and cell lines. Loss- and gain-of-function studies demonstrated that circNF1 significantly promotes cell proliferation. Furthermore, luciferase reporter assays showed that circNF1 binds to miR-16, thereby derepressing its downstream target mRNAs, MAP7 and AKT3. Targeted silencing or overexpression of circNF1 had no effect on levels of its linear RNA counterpart, NF1. Taken together, these results suggest that circNF1 acts as a novel oncogenic circRNA in GC by functioning as a miR-16 sponge.
Identifiants
pubmed: 30576282
doi: 10.1530/ERC-18-0478
pii: ERC-18-0478.R1
pmc: PMC6545167
mid: NIHMS1517668
doi:
pii:
Substances chimiques
MIRN16 microRNA, human
0
MicroRNAs
0
RNA, Circular
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
265-277Subventions
Organisme : NCI NIH HHS
ID : R01 CA190040
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK118250
Pays : United States
Organisme : NCI NIH HHS
ID : UG3 CA211457
Pays : United States
Organisme : NCI NIH HHS
ID : UH3 CA211457
Pays : United States
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