Nucleocytoplasmic shuttling of the human parainfluenza virus type 2 phosphoprotein.
Active Transport, Cell Nucleus
Animals
Cell Line
Cell Nucleus
/ metabolism
Chlorocebus aethiops
Fatty Acids, Unsaturated
/ pharmacology
HeLa Cells
Humans
Karyopherins
/ metabolism
Nuclear Export Signals
Nuclear Localization Signals
/ metabolism
Parainfluenza Virus 2, Human
/ metabolism
Phosphoproteins
/ genetics
Protein Transport
Vero Cells
Viral Proteins
/ genetics
Human parainfluenza virus type 2
Nuclear export
Nuclear import
P protein
V protein
Journal
Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
20
08
2018
revised:
21
11
2018
accepted:
05
12
2018
pubmed:
24
12
2018
medline:
21
3
2019
entrez:
22
12
2018
Statut:
ppublish
Résumé
Human parainfluenza virus type 2 phosphoprotein (P) is an essential component of viral polymerase. The P gene encodes both P and accessory V proteins by a specific gene editing mechanism. Therefore, the N-terminal 164 amino acids of P protein are common to V protein. Interestingly, while P protein is located in the cytoplasm, V protein is found mainly in the nucleus. Using deletion mutants, we show the presence of a nuclear localization signal (NLS) in the P/V common domain, and a nuclear export signal (NES) in the C-terminal P specific region. The NLS region makes a complex with importin α5 or 7. In the presence of leptomycin B, P protein is retained in the nucleus, indicating that it contains a CRM1-dependent NES. We identified the NLS (
Identifiants
pubmed: 30576860
pii: S0042-6822(18)30369-6
doi: 10.1016/j.virol.2018.12.005
pii:
doi:
Substances chimiques
Fatty Acids, Unsaturated
0
Karyopherins
0
Nuclear Export Signals
0
Nuclear Localization Signals
0
P protein, Human parainfluenza virus 2
0
Phosphoproteins
0
V protein, human parainfluenza virus type 1
0
Viral Proteins
0
leptomycin B
Y031I2N1EO
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
54-63Informations de copyright
Copyright © 2018 Elsevier Inc. All rights reserved.