Curvature-dependent constraints drive remodeling of epithelia.
Alginates
/ chemistry
Animals
Biomechanical Phenomena
Cell Adhesion
Cell Culture Techniques
Cell Line
Cell Movement
Cells, Immobilized
Collagen
/ chemistry
Dogs
Drug Combinations
Epithelial Cells
/ metabolism
Laminin
/ chemistry
Madin Darby Canine Kidney Cells
Mechanotransduction, Cellular
Mice
Organ Specificity
Proteoglycans
/ chemistry
3D cell culture
Bioengineering
Cylindrical confinement
Epithelial morphogenesis
Journal
Journal of cell science
ISSN: 1477-9137
Titre abrégé: J Cell Sci
Pays: England
ID NLM: 0052457
Informations de publication
Date de publication:
24 01 2019
24 01 2019
Historique:
received:
11
07
2018
accepted:
17
12
2018
pubmed:
24
12
2018
medline:
6
2
2020
entrez:
23
12
2018
Statut:
epublish
Résumé
Epithelial tissues function as barriers that separate the organism from the environment. They usually have highly curved shapes, such as tubules or cysts. However, the processes by which the geometry of the environment and the cell's mechanical properties set the epithelium shape are not yet known. In this study, we encapsulated two epithelial cell lines, MDCK and J3B1A, into hollow alginate tubes and grew them under cylindrical confinement forming a complete monolayer. MDCK monolayers detached from the alginate shell at a constant rate, whereas J3B1A monolayers detached at a low rate unless the tube radius was reduced. We showed that this detachment is driven by contractile stresses in the epithelium and can be enhanced by local curvature. This allows us to conclude that J3B1A cells exhibit smaller contractility than MDCK cells. Monolayers inside curved tubes detach at a higher rate on the outside of a curve, confirming that detachment is driven by contraction.
Identifiants
pubmed: 30578312
pii: jcs.222372
doi: 10.1242/jcs.222372
pmc: PMC6398478
pii:
doi:
Substances chimiques
Alginates
0
Drug Combinations
0
Laminin
0
Proteoglycans
0
matrigel
119978-18-6
Collagen
9007-34-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Research Council
ID : 311536
Pays : International
Informations de copyright
© 2019. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing or financial interests.
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