Genetic modification of scAAV-equine-BMP-2 transduced bone-marrow-derived mesenchymal stem cells before and after cryopreservation: An "off-the-shelf" option for fracture repair.
bone biology
bone fracture
bone repair
progenitors and stem cells
therapeutics
Journal
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
ISSN: 1554-527X
Titre abrégé: J Orthop Res
Pays: United States
ID NLM: 8404726
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
17
07
2018
accepted:
12
12
2018
pubmed:
24
12
2018
medline:
21
12
2019
entrez:
23
12
2018
Statut:
ppublish
Résumé
Optimizing the environment of complex bone healing and improving treatment of catastrophic bone fractures and segmental bone defects remains an unmet clinical need both human and equine veterinary medical orthopaedics. The objective of this study was to determine whether scAAV-equine-BMP-2 transduced cells would induce osteogenesis in equine bone marrow derived mesenchymal stem cells (BMDMSCs) in vitro, and if these cells could be cryopreserved in an effort to osteogenically prime them as an "off-the-shelf" gene therapeutic approach for fracture repair. Our study found that transgene expression is altered by cell expansion, as would be expected by a transduction resulting in episomal transgene expression, and that osteoinductive levels could still be achieved 5 days after recovery, and protein expression would continue up to 14 days after transduction. This is the first evidence that cryopreservation of genetically modified BMDMSCs would not alter the osteoinductive potential or clinical use of allogeneic donor cells in cases of equine fracture repair. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:1310-1317, 2019.
Identifiants
pubmed: 30578639
doi: 10.1002/jor.24209
pmc: PMC8366205
mid: NIHMS1730238
doi:
Substances chimiques
Bone Morphogenetic Protein 2
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1310-1317Subventions
Organisme : NIH HHS
ID : T32 OD012201
Pays : United States
Informations de copyright
© 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
Références
Gene Ther. 2015 Jun;22(6):467-75
pubmed: 25809463
Biomaterials. 2009 May;30(14):2816-25
pubmed: 19232714
J Bone Joint Surg Am. 2006 Feb;88(2):355-65
pubmed: 16452748
Curr Stem Cell Res Ther. 2014;9(6):497-503
pubmed: 25175766
Front Vet Sci. 2015 Dec 07;2:67
pubmed: 26664993
Acta Pharmacol Sin. 2013 May;34(5):699-709
pubmed: 23564084
Spine (Phila Pa 1976). 2014 Apr 20;39(9):695-700
pubmed: 24503679
Mol Ther. 2008 Oct;16(10):1648-56
pubmed: 18682697
Gene Ther. 2000 May;7(9):734-9
pubmed: 10822299
Gene Ther. 2015 Jul;22(7):536-45
pubmed: 25902762
J Orthop Res. 2015 Oct;33(10):1455-65
pubmed: 25418909
Int Orthop. 2014 Sep;38(9):1761-9
pubmed: 25038968
Equine Vet J Suppl. 2011 Nov;(40):7-11
pubmed: 22082439
Clin J Am Soc Nephrol. 2008 Nov;3 Suppl 3:S131-9
pubmed: 18988698
Mol Ther. 2011 Aug;19(8):1416-25
pubmed: 21206485
J Orthop Res. 2007 May;25(5):665-77
pubmed: 17290432
Sci Rep. 2014 Jul 29;4:5861
pubmed: 25069854
Expert Rev Mol Med. 2010 Jun 23;12:e18
pubmed: 20569532
Health Technol Assess. 2007 Aug;11(30):1-150, iii-iv
pubmed: 17669279
J Am Acad Orthop Surg. 2018 Jan 15;26(2):e36-e38
pubmed: 29303924
Vet Immunol Immunopathol. 2013 Nov 15;156(1-2):99-106
pubmed: 24094688
J Anim Sci Biotechnol. 2013 Oct 29;4(1):40
pubmed: 24169030
Invest Ophthalmol Vis Sci. 2010 Jan;51(1):236-48
pubmed: 19684004
Orthopedics. 2012 Jun;35(6):e847-54
pubmed: 22691656
Vet Res Commun. 2014 Mar;38(1):73-80
pubmed: 24442646
J Gene Med. 2009 Jul;11(7):605-14
pubmed: 19384892
Immunol Cell Biol. 2013 Jan;91(1):40-51
pubmed: 23207278
Stem Cells Cloning. 2008 Nov 02;1:1-9
pubmed: 24198500
J Orthop Res. 2006 Jun;24(6):1279-91
pubmed: 16649180
Vet Surg. 2010 Jul;39(5):588-93
pubmed: 20459481
Biochem Biophys Res Commun. 2002 Mar 22;292(1):144-52
pubmed: 11890685
Mol Ther. 2011 May;19(5):960-8
pubmed: 21343916
Bone. 2007 Apr;40(4):931-8
pubmed: 17236835
Bone. 2006 Jul;39(1):61-71
pubmed: 16488673
Stem Cell Res Ther. 2017 Apr 18;8(1):80
pubmed: 28420427
Gene Ther. 2014 Feb;21(2):139-47
pubmed: 24285218
Stem Cells Dev. 2017 Apr 1;26(7):503-511
pubmed: 27958776
J Bone Joint Surg Am. 1999 Jul;81(7):905-17
pubmed: 10428121
J Orthop Res. 2018 Jun;36(6):1559-1572
pubmed: 29280510
Vet Comp Orthop Traumatol. 2013;26(6):453-60
pubmed: 24080668
Vet Surg. 2012 Apr;41(3):345-54
pubmed: 22308976
Curr Stem Cell Res Ther. 2014;9(6):452-7
pubmed: 24548143
Eur Cell Mater. 2009 Dec 31;18:96-111
pubmed: 20073015
Stem Cell Res Ther. 2014 Jan 24;5(1):13
pubmed: 24461709
J Orthop Res. 2010 Oct;28(10):1330-7
pubmed: 20309952