Interplay between Membrane Curvature and Cholesterol: Role of Palmitoylated Caveolin-1.
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
08 01 2019
08 01 2019
Historique:
received:
25
06
2018
revised:
14
10
2018
accepted:
01
11
2018
pubmed:
24
12
2018
medline:
3
1
2020
entrez:
24
12
2018
Statut:
ppublish
Résumé
Caveolin-1 (cav-1) is an important player in cell signaling and endocytosis that has been shown to colocalize with cholesterol-rich membrane domains. Experimental studies with varying cav-1 constructs have suggested that it can induce both cholesterol clustering and membrane curvature. Here, we probe the molecular origin of membrane curvature and cholesterol clustering by cav-1 by using coarse-grain molecular dynamics simulations. We have performed a series of simulations of a functionally important cav-1 construct, comprising the membrane-interacting domains and a C-terminal palmitoyl tail. Our results suggest that cav-1 is able to induce cholesterol clustering in the membrane leaflet to which it is bound as well as the opposing leaflet. A positive membrane curvature is observed upon cav-1 binding in cholesterol-containing bilayers. Interestingly, we observe an interplay between cholesterol clustering and membrane curvature such that cav-1 is able to induce higher membrane curvature in cholesterol-rich membranes. The role of the cav-1 palmitoyl tail is less clear and appears to increase the membrane contacts. Further, we address the importance of the secondary structure of cav-1 domains and show that it could play an important role in membrane curvature and cholesterol clustering. Our work is an important step toward a molecular picture of caveolae and vesicular endocytosis.
Identifiants
pubmed: 30579563
pii: S0006-3495(18)34449-7
doi: 10.1016/j.bpj.2018.11.3127
pmc: PMC6342733
pii:
doi:
Substances chimiques
Caveolin 1
0
Lipid Bilayers
0
Cholesterol
97C5T2UQ7J
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
69-78Informations de copyright
Copyright © 2018 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Références
J Am Chem Soc. 2012 Jan 25;134(3):1543-52
pubmed: 22191854
Biophys J. 2012 Sep 5;103(5):959-69
pubmed: 23009845
Curr Top Membr. 2015;75:305-36
pubmed: 26015287
J Chem Phys. 2018 May 14;148(18):184702
pubmed: 29764126
Science. 1995 Apr 14;268(5208):221-5
pubmed: 7716512
Biophys J. 2015 Aug 4;109(3):564-73
pubmed: 26244738
Proc Natl Acad Sci U S A. 1995 Oct 24;92(22):10339-43
pubmed: 7479780
J Comput Chem. 2005 Dec;26(16):1701-18
pubmed: 16211538
Chembiochem. 2011 May 2;12(7):1049-55
pubmed: 21433241
J Phys Chem B. 2012 Nov 1;116(43):12991-6
pubmed: 23067252
Sci Rep. 2016 Apr 05;6:23981
pubmed: 27046665
J Chem Theory Comput. 2008 May;4(5):819-34
pubmed: 26621095
Biophys J. 2014 Mar 18;106(6):1371-80
pubmed: 24655512
Biophys J. 2010 Dec 1;99(11):3666-74
pubmed: 21112291
J Phys Chem B. 2012 Dec 20;116(50):14556-64
pubmed: 23199331
J Am Chem Soc. 2008 Jan 9;130(1):10-1
pubmed: 18076174
Biophys J. 2015 Oct 20;109(8):1686-8
pubmed: 26488659
Biochim Biophys Acta. 2009 Jan;1788(1):149-68
pubmed: 19013128
Biophys J. 2008 Sep;95(5):2325-39
pubmed: 18515373
J Biol Chem. 2000 Jul 14;275(28):21605-17
pubmed: 10801850
J Cell Biol. 2016 Aug 15;214(4):375-87
pubmed: 27528656
Biochemistry. 2014 Jul 15;53(27):4320-2
pubmed: 24960539
Sci Rep. 2016 Sep 21;6:33702
pubmed: 27650365
J Biol Chem. 1998 Mar 6;273(10):5419-22
pubmed: 9488658
J Med Chem. 2007 Feb 22;50(4):674-84
pubmed: 17263520
Biophys J. 2010 Oct 6;99(7):2134-42
pubmed: 20923647
Biophys J. 2007 May 15;92(10):3595-602
pubmed: 17325001
Sci Rep. 2017 Nov 22;7(1):16078
pubmed: 29167583
Biochim Biophys Acta. 2012 Jan;1818(1):12-8
pubmed: 21958667
Nat Methods. 2015 Jan;12(1):7-8
pubmed: 25549265
FEBS Lett. 2006 Oct 2;580(22):5301-5
pubmed: 16979631
J Am Chem Soc. 2007 Oct 10;129(40):12280-6
pubmed: 17880077
Biochim Biophys Acta. 2010 Feb;1798(2):275-85
pubmed: 19819220
Biochim Biophys Acta. 2014 Oct;1838(10):2588-99
pubmed: 24998359
Proc Natl Acad Sci U S A. 1995 Sep 26;92(20):9407-11
pubmed: 7568142
Eur Biophys J. 2010 Jan;39(2):307-25
pubmed: 19847421
Physiol Rev. 2014 Oct;94(4):1219-48
pubmed: 25287863
J Chem Phys. 2014 Mar 28;140(12):124905
pubmed: 24697479
J Mol Biol. 2005 Jan 14;345(2):339-50
pubmed: 15571726
Biophys J. 2015 Nov 17;109(10):2067-78
pubmed: 26588566
EMBO J. 1993 Apr;12(4):1597-605
pubmed: 8385608
Science. 2004 Jan 23;303(5657):495-9
pubmed: 14645856
Mol Biol Cell. 1995 Jul;6(7):911-27
pubmed: 7579702
Chem Phys Lipids. 2018 Mar;211:93-99
pubmed: 29162371
Biophys J. 2004 Dec;87(6):3894-900
pubmed: 15377528
Biophys J. 2008 Aug;95(4):1866-76
pubmed: 18469070
Biochim Biophys Acta. 2012 May;1818(5):1158-64
pubmed: 22240009
Genome Biol. 2004;5(3):214
pubmed: 15003112
Proc Natl Acad Sci U S A. 2016 Oct 4;113(40):11226-11231
pubmed: 27655892
Biophys J. 2009 Nov 18;97(10):2746-53
pubmed: 19917228
Biophys J. 2007 Apr 15;92(8):2819-30
pubmed: 17259270
Biochemistry. 2012 Jan 10;51(1):90-9
pubmed: 22142403
Faraday Discuss. 2013;161:347-63; discussion 419-59
pubmed: 23805749
J Biol Chem. 1999 Aug 6;274(32):22660-7
pubmed: 10428847
J Biol Chem. 1995 Mar 24;270(12):6838-42
pubmed: 7896831
Front Mol Biosci. 2015 Nov 18;2:66
pubmed: 26636093
Angew Chem Int Ed Engl. 2007;46(43):8234-7
pubmed: 17886310
Oncogene. 1998 Mar;16(11):1391-7
pubmed: 9525738
Biophys J. 2015 Dec 15;109(12):2467-2470
pubmed: 26682805
Biopolymers. 2015 Jan;104(1):11-20
pubmed: 25471446
Mol Cell Biol. 1999 Nov;19(11):7289-304
pubmed: 10523618
Biochemistry. 2010 Sep 7;49(35):7485-93
pubmed: 20669961
Biophys J. 2004 Jun;86(6):3556-75
pubmed: 15189854
PLoS One. 2012;7(6):e38302
pubmed: 22761674
Methods Mol Biol. 1999;116:51-60
pubmed: 10399145
J Chem Theory Comput. 2015 May 12;11(5):2278-91
pubmed: 26574426
Chem Soc Rev. 2018 May 21;47(10):3470-3489
pubmed: 29688238
PLoS One. 2013 Jul 26;8(7):e71018
pubmed: 23923044
Cell. 2012 Mar 30;149(1):124-36
pubmed: 22464325
J Am Chem Soc. 2003 Sep 17;125(37):11144-5
pubmed: 16220905
Eur Biophys J. 2016 Sep;45(6):511-21
pubmed: 27038819
Soft Matter. 2013 Dec 21;9(47):
pubmed: 24358048
Biochim Biophys Acta. 2000 Dec 15;1529(1-3):210-22
pubmed: 11111090
Phys Chem Chem Phys. 2015 Jan 14;17(2):1390-8
pubmed: 25427292
J Biophys Biochem Cytol. 1955 Sep 25;1(5):445-58
pubmed: 13263332
J Am Chem Soc. 2004 Nov 24;126(46):15277-86
pubmed: 15548025
J Am Chem Soc. 2010 May 19;132(19):6710-8
pubmed: 20411937
J Biol Chem. 2010 Oct 22;285(43):33371-80
pubmed: 20729193
Biophys J. 2007 Oct 15;93(8):2697-712
pubmed: 17557790
J Am Chem Soc. 2009 Jul 8;131(26):9166-7
pubmed: 19530654
J Chem Phys. 2007 Jan 7;126(1):014101
pubmed: 17212484
Phys Chem Chem Phys. 2009 Mar 28;11(12):2056-67
pubmed: 19280016
Proc Natl Acad Sci U S A. 2012 May 22;109(21):8097-102
pubmed: 22562795
Cell. 1992 Feb 21;68(4):673-82
pubmed: 1739974
J Comput Aided Mol Des. 2013 Oct;27(10):845-58
pubmed: 24150904
PLoS Comput Biol. 2013;9(1):e1002892
pubmed: 23382665
J Am Chem Soc. 2012 Oct 17;134(41):17278-85
pubmed: 22994893
J Biol Chem. 1997 Mar 7;272(10):6525-33
pubmed: 9045678
Biophys J. 2008 May 15;94(10):3976-86
pubmed: 18227127
Curr Opin Cell Biol. 2010 Aug;22(4):430-6
pubmed: 20605711
J Phys Chem B. 2007 Jul 12;111(27):7812-24
pubmed: 17569554
ScientificWorldJournal. 2013 May 16;2013:965230
pubmed: 23766730
Nat Commun. 2014 Sep 15;5:4916
pubmed: 25222832