Analysis of antibody responses to selected Plasmodium falciparum merozoite surface antigens in mild and cerebral malaria and associations with clinical outcomes.
Adolescent
Adult
Aged
Antibodies, Protozoan
/ blood
Antigens, Protozoan
/ immunology
Cell Extracts
/ immunology
Child
Child, Preschool
Disease Progression
Female
Hospitalization
Humans
Immunoglobulin M
/ blood
Infant
Malaria, Cerebral
/ immunology
Malaria, Falciparum
/ immunology
Male
Merozoite Surface Protein 1
/ immunology
Merozoites
/ immunology
Middle Aged
Plasmodium falciparum
/ immunology
Recombinant Proteins
/ immunology
Survival Analysis
Treatment Outcome
Urban Population
Young Adult
IgG subclasses
antibodies
cerebral malaria
merozoite surface protein
Journal
Clinical and experimental immunology
ISSN: 1365-2249
Titre abrégé: Clin Exp Immunol
Pays: England
ID NLM: 0057202
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
accepted:
13
12
2018
pubmed:
24
12
2018
medline:
18
2
2020
entrez:
24
12
2018
Statut:
ppublish
Résumé
Merozoite surface proteins (MSPs) are critical for parasite invasion; they represent attractive targets for antibody-based protection against clinical malaria. To identify protection-associated target MSPs, the present study analysed antibody responses to whole merozoite extract (ME) and to defined MSP recombinant antigens in hospitalized patients from a low endemic urban area as a function of disease severity (mild versus cerebral malaria). Sera from 110 patients with confirmed severe cerebral malaria (CM) and 91 patients with mild malaria (MM) were analysed (mean age = 29 years) for total and subclass immunoglobulin (Ig)G to ME and total IgG to MSP1p19, MSP2, MSP3, MSP4 and MSP5 by enzyme-linked immunosorbent assay (ELISA). Functional antibody responses were evaluated using the antibody-dependent respiratory burst (ADRB) assay in a subset of sera. There was a trend towards higher IgG1 and IgG4 levels to ME in CM compared to MM; only ME IgM responses differed significantly between fatal and surviving CM patients. Increased prevalence of IgG to individual MSPs was found in the CM compared to the MM group, including significantly higher levels of IgG to MSP4 and MSP5 in the former. Sera from fatal (24·5%) versus surviving cases showed significantly lower IgG to MSP1p19 and MSP3 (P < 0·05). ADRB assay readouts correlated with high levels of anti-MSP IgG, and trended higher in sera from patients with surviving compared to fatal CM outcome (P = 0·07). These results document strong differential antibody responses to MSP antigens as targets of protective immunity against CM and in particular MSP1p19 and MSP3 as prognostic indicators.
Identifiants
pubmed: 30580455
doi: 10.1111/cei.13254
pmc: PMC6422657
doi:
Substances chimiques
Antibodies, Protozoan
0
Antigens, Protozoan
0
Cell Extracts
0
Immunoglobulin M
0
Merozoite Surface Protein 1
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
86-96Informations de copyright
© 2018 British Society for Immunology.
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