Real-Time Insights into Biological Events: In-Cell Processes and Protein-Ligand Interactions.
Batch Cell Culture Techniques
/ instrumentation
Bioreactors
Carbonic Anhydrase II
/ antagonists & inhibitors
Drug Discovery
/ methods
Enzyme Inhibitors
/ pharmacology
HEK293 Cells
Humans
Magnetic Resonance Spectroscopy
/ instrumentation
Protein Binding
Superoxide Dismutase
/ antagonists & inhibitors
Journal
Biophysical journal
ISSN: 1542-0086
Titre abrégé: Biophys J
Pays: United States
ID NLM: 0370626
Informations de publication
Date de publication:
22 01 2019
22 01 2019
Historique:
received:
06
09
2018
revised:
30
10
2018
accepted:
27
11
2018
pubmed:
26
12
2018
medline:
3
1
2020
entrez:
25
12
2018
Statut:
ppublish
Résumé
FlowNMR has the aim of continuously monitoring processes that occur in conditions that are not compatible with being carried out within a closed tube. However, it is sample intensive and not suitable for samples, such as proteins or living cells, that are often available in limited volumes and possibly low concentrations. We here propose a dialysis-based modification of a commercial flowNMR setup that allows for recycling the medium while confining the sample (proteins and cells) within the active volume of the tube. This approach is demonstrated in the specific cases of in-cell NMR and protein-based ligand studies.
Identifiants
pubmed: 30580921
pii: S0006-3495(18)34454-0
doi: 10.1016/j.bpj.2018.11.3132
pmc: PMC6350048
pii:
doi:
Substances chimiques
Enzyme Inhibitors
0
Superoxide Dismutase
EC 1.15.1.1
Carbonic Anhydrase II
EC 4.2.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
239-247Informations de copyright
Copyright © 2018 Biophysical Society. Published by Elsevier Inc. All rights reserved.
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