A lethal pneumonia model of Acinetobacter baumannii: an investigation in immunocompetent mice.
Acinetobacter Infections
/ immunology
Acinetobacter baumannii
/ immunology
Animals
Bacterial Load
/ immunology
Bacterial Vaccines
/ immunology
Bronchoalveolar Lavage Fluid
/ microbiology
Cytokines
/ biosynthesis
Disease Models, Animal
Flow Cytometry
Humans
Lung
/ immunology
Male
Mice
Mice, Inbred C57BL
Neutrophils
/ immunology
Pneumonia, Bacterial
/ microbiology
RNA, Messenger
/ biosynthesis
Vaccination
Acinetobacter baumannii
Animal models
Hypervirulent strain
Immunocompetent mice
Pneumonia
Journal
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
ISSN: 1469-0691
Titre abrégé: Clin Microbiol Infect
Pays: England
ID NLM: 9516420
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
16
09
2018
revised:
26
11
2018
accepted:
15
12
2018
pubmed:
26
12
2018
medline:
18
7
2019
entrez:
25
12
2018
Statut:
ppublish
Résumé
Acinetobacter baumannii can cause severe nosocomial and community-acquired pneumonia. To study the pathogenesis of A. baumannii and to develop new treatments, appropriate mouse models are needed. Most reported mouse models of pulmonary A. baumannii infection are non-lethal or require mouse immunosuppression to enhance infection. These models are not suitable for studying host immune responses or evaluating immunotherapies. The virulence of 30 clinical isolates was assessed in mice. The most virulent isolate, SJZ24, was selected to develop a pneumonia model in immunocompetent mice. The cytokine mRNA expression in the lung was assessed with real-time PCR. The cell infiltration in bronchoalveolar lavage fluid (BALF) after SJZ24 infection was determined by flow cytometry. Vaccine efficacy was assessed using this model. Intratracheal inoculation of SJZ24 (5 × 10 We established a lethal pneumonia model in immunocompetent mice with hypervirulent A. baumannii isolate SJZ24. This model can be used to study the immune response to A. baumannii infection and to evaluate vaccine efficacy.
Identifiants
pubmed: 30583061
pii: S1198-743X(18)30809-7
doi: 10.1016/j.cmi.2018.12.020
pii:
doi:
Substances chimiques
Bacterial Vaccines
0
Cytokines
0
RNA, Messenger
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
516.e1-516.e4Informations de copyright
Copyright © 2018 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.