Human antibodies eluted from ligand-free Sepharose capable of binding bacterial polysaccharides and sulfated glycans.

Antibodies, Bacterial / blood Humans Polysaccharides, Bacterial / chemistry Sepharose / chemistry
Agarose Antibodies Bacteria Polysaccharides Printed glycan array Sepharose

Journal

Molecular immunology
ISSN: 1872-9142
Titre abrégé: Mol Immunol
Pays: England
ID NLM: 7905289

Informations de publication

Date de publication:
02 2019
Historique:
received: 26 03 2018
revised: 30 11 2018
accepted: 09 12 2018
pubmed: 26 12 2018
medline: 1 5 2019
entrez: 25 12 2018
Statut: ppublish

Résumé

Sepharose matrix without immobilized ligands binds antibodies from human blood serum or immunoglobulin preparations. The eluted antibodies bind bacterial polysaccharides having no structural similarity to agarose (Sepharose is a cross-linked polysaccharide agarose) with a high affinity. It is concluded that the identified antibodies are capable of recognizing spatial rather than linear epitopes of bacterial polysaccharides. This side activity of Sepharose matrix should be taken into account in isolating target antibodies and other proteins from human blood.

Identifiants

DOI: 10.1016/j.molimm.2018.12.011 PMID: 30583222
pubmed: 30583222
pii: S0161-5890(18)31015-0
doi: 10.1016/j.molimm.2018.12.011
pii:
doi:

Substances chimiques

Antibodies, Bacterial 0
Polysaccharides, Bacterial 0
Sepharose 9012-36-6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

63-68

Informations de copyright

Copyright © 2018 Elsevier Ltd. All rights reserved.

Auteurs

K L Dobrochaeva (KL)

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 ul. Miklukho-Maklaya, Moscow, 117997, Russian Federation.

N R Khasbiullina (NR)

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 ul. Miklukho-Maklaya, Moscow, 117997, Russian Federation; Semiotik LLC, Moscow, Russian Federation.

N V Shilova (NV)

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 ul. Miklukho-Maklaya, Moscow, 117997, Russian Federation.

P S Obukhova (PS)

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 ul. Miklukho-Maklaya, Moscow, 117997, Russian Federation.

Yu A Knirel (YA)

N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninsky pr., Moscow, 119991, Russian Federation.

A Yu Nokel (AY)

Semiotik LLC, Moscow, Russian Federation.

N V Bovin (NV)

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 ul. Miklukho-Maklaya, Moscow, 117997, Russian Federation; School of Engineering, Computer & Mathematical Sciences, Auckland University of Technology, Auckland 1010, New Zealand. Electronic address: professorbovin@yandex.ru.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps antibactériens › Human antibodies eluted from ligand-free...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Human antibodies eluted from ligand-free...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes bactériens › Polyosides bactériens › Human antibodies eluted from ligand-free...
questionsmedicales.fr › Glucides › Polyosides › Agarose › Human antibodies eluted from ligand-free...