Testicular Fine-Needle Aspiration for Sperm Retrieval in Azoospermia: A Small Step toward the Technical Standardization.

Azoospermia Biopsy, fine-needle Infertility, male Reproductive techniques Sperm retrieval

Journal

The world journal of men's health
ISSN: 2287-4208
Titre abrégé: World J Mens Health
Pays: Korea (South)
ID NLM: 101596899

Informations de publication

Date de publication:
Jan 2019
Historique:
received: 31 08 2018
revised: 23 10 2018
accepted: 24 10 2018
entrez: 26 12 2018
pubmed: 26 12 2018
medline: 26 12 2018
Statut: ppublish

Résumé

The aim was to describe our preliminary experience performing testicular fine-needle aspiration (TEFNA) with a larger needle in infertile patients with obstructive azoospermia, and to provide a systematic literature review of the different testicular sperm aspiration techniques, according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement recommendations. We prospectively collected data between March 2017 and June 2018. All men underwent bilateral TEFNA under analgo-sedation, using a larger disposable 18-gauge butterfly needle with 60 mL Luer-Lock syringe attached to it. Thirty consecutive patients were enrolled. Median operative time was 16 minutes (interquartile range [IQR]: 12-30 minutes). No intraoperative complications occurred. Two/thirty patients (6.7%) reported postoperative adverse events: 1 patient had prolonged orchialgia, 1 patient presented scrotal hematoma. Successful sperm retrieval was found in 28/30 cases (93.3%). Median sperm concentration was 0.05 ×10⁶/mL (IQR: 0.001-0.1 ×10⁶/mL). Median total sperm motility was 10% (IQR: 0%-15%). In 20/30 men (66.7%) sperm retrieved was used for fresh intracytoplasmic sperm injection cycle, in 8/30 (26.7%) sperm cryopreservation was necessary, because on the day of sperm retrieval the female resulted not responder to ovarian stimulation. In this cases mean number of 3 (IQR: 1-4) bio system straws was cryopreserved. TEFNA with 18-gauge needle proved to be a feasible, safe and effective treatment, even if future prospective studies will be addressed to clarify what type of azoospermia benefits from this procedure, and if a larger needle permits to improve Assisted Reproductive Technologies (ART) outcomes.

Identifiants

pubmed: 30584991
pii: 37.55
doi: 10.5534/wjmh.180077
pmc: PMC6305866
doi:

Types de publication

Journal Article

Langues

eng

Pagination

55-67

Informations de copyright

Copyright © 2019 Korean Society for Sexual Medicine and Andrology.

Déclaration de conflit d'intérêts

The authors have no potential conflicts of interest to disclose.

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Auteurs

Gianmartin Cito (G)

Department of Urology, Careggi Hospital, University of Florence, Florence, Italy.

Maria Elisabetta Coccia (ME)

Assisted Reproductive Technology Center, Careggi Hospital, University of Florence, Florence, Italy.

Francesco Sessa (F)

Department of Urology, Careggi Hospital, University of Florence, Florence, Italy.

Andrea Cocci (A)

Department of Urology, Careggi Hospital, University of Florence, Florence, Italy. cocci.andrea@gmail.com.

Pierangelo Verrienti (P)

Department of Urology, Careggi Hospital, University of Florence, Florence, Italy.

Rita Picone (R)

Assisted Reproductive Technology Center, Careggi Hospital, University of Florence, Florence, Italy.

Rossella Fucci (R)

Assisted Reproductive Technology Center, Careggi Hospital, University of Florence, Florence, Italy.

Luciana Criscuoli (L)

Assisted Reproductive Technology Center, Careggi Hospital, University of Florence, Florence, Italy.

Sergio Serni (S)

Department of Urology, Careggi Hospital, University of Florence, Florence, Italy.

Marco Carini (M)

Department of Urology, Careggi Hospital, University of Florence, Florence, Italy.

Alessandro Natali (A)

Department of Urology, Careggi Hospital, University of Florence, Florence, Italy.

Classifications MeSH