Competitive binding of monoclonal antibody monomer-dimer mixtures on ceramic hydroxyapatite.
Antibodies
Competitive binding
Hydroxyapatite
Mass transfer
Monomer and dimer
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
22 Feb 2019
22 Feb 2019
Historique:
received:
12
10
2018
revised:
06
12
2018
accepted:
11
12
2018
pubmed:
28
12
2018
medline:
5
3
2019
entrez:
28
12
2018
Statut:
ppublish
Résumé
The internal structure of ceramic hydroxyapatite CHT Type I and Type II and the adsorption behavior of a monoclonal antibody in monomeric and dimeric forms are determined. Both CHT types contain elongated nanocrystals with size about 20 x 100 nm for Type I and about 50 x 200 nm for Type II. Internal porosities and apparent pore radii based on inverse size exclusion chromatography are 0.73 and 30 nm for Type I and 0.70 and 49 nm for Type II. Adsorption isotherms show maximum capacities of 95 and 110 mg/mL of particle for monomer and dimer on Type I and of 55 and 67 mg/mL of particle on Type II, in approximate agreement with the ratio of surface areas. The isotherms are dependent on the Na
Identifiants
pubmed: 30587350
pii: S0021-9673(18)31530-9
doi: 10.1016/j.chroma.2018.12.023
pii:
doi:
Substances chimiques
Antibodies, Monoclonal
0
Polymers
0
Durapatite
91D9GV0Z28
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
136-145Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.