Mesenchymal stromal cells from infants with simple polydactyly modulate immune responses more efficiently than adult mesenchymal stromal cells.
Adult
Age Factors
Cell Differentiation
/ immunology
Cell Proliferation
Cells, Cultured
Coculture Techniques
Connective Tissue Cells
/ physiology
Female
Humans
Infant
Interleukin-10
/ biosynthesis
Lymphocyte Culture Test, Mixed
Male
Mesenchymal Stem Cells
/ immunology
Middle Aged
Polydactyly
/ pathology
Tumor Necrosis Factor-alpha
/ biosynthesis
T cells
immunomodulation
interleukin-10
mesenchymal stromal cells
tumor necrosis factor-α
Journal
Cytotherapy
ISSN: 1477-2566
Titre abrégé: Cytotherapy
Pays: England
ID NLM: 100895309
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
28
06
2018
revised:
04
11
2018
accepted:
29
11
2018
pubmed:
1
1
2019
medline:
25
2
2020
entrez:
1
1
2019
Statut:
ppublish
Résumé
Bone marrow-derived stromal cells or mesenchymal stromal cells (BMSCs or MSCs, as we will call them in this work) are multipotent progenitor cells that can differentiate into osteoblasts, adipocytes and chondrocytes. In addition, MSCs have been shown to modulate the function of a variety of immune cells. Donor age has been shown to affect the regenerative potential, differentiation, proliferation and anti-inflammatory potency of MSCs; however, the impact of donor age on their immunosuppressive activity is unknown. In this study, we evaluated the ability of MSCs derived from very young children and adults on T-cell suppression and cytokine secretion by monocytes/macrophages. MSCs were obtained from extra digits of children between 10 and 21 months and adults between 28 and 64 years of age. We studied cell surface marker expression, doubling time, lineage differentiation potential and immunosuppressive function of the MSCs. Young MSCs double more quickly and differentiate into bone and fat cells more efficiently than those from older donors. They also form more and dense colonies of fibroblasts (colony forming unit-fibroblast [CFU-F]). MSCs from both young and adult subjects suppressed T-cell proliferation in a mitogen-induced assay at 1:3 and 1:30 ratios. At a 1:30 ratio, however, MSCs from adults did not, but MSCs from infants did suppress T-cell proliferation. In the mixed lymphocyte reaction assay, MSCs from infants produced similar levels of suppression at all three MSC/T-cell ratios, but adult MSCs only inhibited T-cell proliferation at a 1:3 ratio. Cytokine analyses of co-cultures of MSCs and macrophages showed that both adult and young MSCs suppress tumor necrosis factor alpha (TNF-α) and induce interleukin-10 (IL-10) production in macrophage co-culture assay in a similar manner. Overall, this work shows that developing MSCs display a higher level of immunosuppression than mature MSCs.
Identifiants
pubmed: 30595353
pii: S1465-3249(18)30698-4
doi: 10.1016/j.jcyt.2018.11.008
pmc: PMC6435420
mid: NIHMS1517552
pii:
doi:
Substances chimiques
IL10 protein, human
0
Tumor Necrosis Factor-alpha
0
Interleukin-10
130068-27-8
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
148-161Subventions
Organisme : Intramural NIH HHS
ID : ZIA DE000714-14
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIC DE000729
Pays : United States
Informations de copyright
Published by Elsevier Inc.
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