Associations of Mitochondrial and Nuclear Mitochondrial Variants and Genes with Seven Metabolic Traits.


Journal

American journal of human genetics
ISSN: 1537-6605
Titre abrégé: Am J Hum Genet
Pays: United States
ID NLM: 0370475

Informations de publication

Date de publication:
03 01 2019
Historique:
received: 01 07 2018
accepted: 06 12 2018
pubmed: 1 1 2019
medline: 5 11 2019
entrez: 1 1 2019
Statut: ppublish

Résumé

Mitochondria (MT), the major site of cellular energy production, are under dual genetic control by 37 mitochondrial DNA (mtDNA) genes and numerous nuclear genes (MT-nDNA). In the CHARGEmtDNA+ Consortium, we studied genetic associations of mtDNA and MT-nDNA associations with body mass index (BMI), waist-hip-ratio (WHR), glucose, insulin, HOMA-B, HOMA-IR, and HbA1c. This 45-cohort collaboration comprised 70,775 (insulin) to 170,202 (BMI) pan-ancestry individuals. Validation and imputation of mtDNA variants was followed by single-variant and gene-based association testing. We report two significant common variants, one in MT-ATP6 associated (p ≤ 5E-04) with WHR and one in the D-loop with glucose. Five rare variants in MT-ATP6, MT-ND5, and MT-ND6 associated with BMI, WHR, or insulin. Gene-based meta-analysis identified MT-ND3 associated with BMI (p ≤ 1E-03). We considered 2,282 MT-nDNA candidate gene associations compiled from online summary results for our traits (20 unique studies with 31 dataset consortia's genome-wide associations [GWASs]). Of these, 109 genes associated (p ≤ 1E-06) with at least 1 of our 7 traits. We assessed regulatory features of variants in the 109 genes, cis- and trans-gene expression regulation, and performed enrichment and protein-protein interactions analyses. Of the identified mtDNA and MT-nDNA genes, 79 associated with adipose measures, 49 with glucose/insulin, 13 with risk for type 2 diabetes, and 18 with cardiovascular disease, indicating for pleiotropic effects with health implications. Additionally, 21 genes related to cholesterol, suggesting additional important roles for the genes identified. Our results suggest that mtDNA and MT-nDNA genes and variants reported make important contributions to glucose and insulin metabolism, adipocyte regulation, diabetes, and cardiovascular disease.

Identifiants

pubmed: 30595373
pii: S0002-9297(18)30452-X
doi: 10.1016/j.ajhg.2018.12.001
pmc: PMC6323610
pii:
doi:

Substances chimiques

DNA, Mitochondrial 0
Glycated Hemoglobin A 0
Insulin 0
Glucose IY9XDZ35W2

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

112-138

Subventions

Organisme : NIDDK NIH HHS
ID : R01 DK078616
Pays : United States
Organisme : NHLBI NIH HHS
ID : K99 HL130580
Pays : United States
Organisme : NIEHS NIH HHS
ID : P30 ES010126
Pays : United States
Organisme : Medical Research Council
ID : MC_PC_19009
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12015/1
Pays : United Kingdom
Organisme : NHLBI NIH HHS
ID : R00 HL130580
Pays : United States
Organisme : Medical Research Council
ID : MR/L01341X/1
Pays : United Kingdom
Organisme : NIDDK NIH HHS
ID : K24 DK080140
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK020541
Pays : United States
Organisme : NHLBI NIH HHS
ID : K01 HL143142
Pays : United States
Organisme : Wellcome Trust
ID : G906919
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : U54 GM115428
Pays : United States
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/F019394/1
Pays : United Kingdom
Organisme : NIDDK NIH HHS
ID : P30 DK079626
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK056341
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL105756
Pays : United States
Organisme : Medical Research Council
ID : MR/S003886/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12015/2
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R023484/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/L01632X/1
Pays : United Kingdom
Organisme : NIDDK NIH HHS
ID : U01 DK078616
Pays : United States
Organisme : Medical Research Council
ID : MC_UU_00007/10
Pays : United Kingdom
Organisme : NIGMS NIH HHS
ID : R01 GM075091
Pays : United States
Organisme : NIDDK NIH HHS
ID : P30 DK056336
Pays : United States
Organisme : Medical Research Council
ID : G9815508
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : NHLBI NIH HHS
ID : R01 HL131573
Pays : United States
Organisme : Wellcome Trust
ID : 208806/Z/17/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12015/5
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/K002767/1
Pays : United Kingdom

Informations de copyright

Copyright © 2018 American Society of Human Genetics. All rights reserved.

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Auteurs

Aldi T Kraja (AT)

Division of Statistical Genomics, Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St Louis, MO 63110, USA. Electronic address: aldikraja@wustl.edu.

Chunyu Liu (C)

Department of Biostatistics, Boston University School of Public Health, Boston, MA 02118, USA.

Jessica L Fetterman (JL)

Evans Department of Medicine and Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA 02118, USA.

Mariaelisa Graff (M)

Department of Epidemiology, University of North Carolina, Chapel Hill, NC 27516, USA.

Christian Theil Have (CT)

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200, Denmark.

Charles Gu (C)

Division of Biostatistics, Washington University School of Medicine, St Louis, MO 63110, USA.

Lisa R Yanek (LR)

GeneSTAR Research Program, Division of General Internal Medicine, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Mary F Feitosa (MF)

Division of Statistical Genomics, Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St Louis, MO 63110, USA.

Dan E Arking (DE)

McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Daniel I Chasman (DI)

Division of Preventive Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA.

Kristin Young (K)

Department of Epidemiology, University of North Carolina, Chapel Hill, NC 27516, USA.

Symen Ligthart (S)

Department of Epidemiology, Erasmus University Medical Center, Rotterdam 3015 CE, the Netherlands.

W David Hill (WD)

Centre for Cognitive Ageing and Cognitive Epidemiology, University of Edinburgh, Department of Psychology, University of Edinburgh, Edinburgh EH8 9JZ, UK.

Stefan Weiss (S)

Interfaculty Institute for Genetics and Functional Genomics, University Medicine and University of Greifswald, Greifswald 17475, Germany.

Jian'an Luan (J)

MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine, Cambridge CB2 0QQ, UK.

Franco Giulianini (F)

Division of Preventive Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA.

Ruifang Li-Gao (R)

Department of Clinical Epidemiology, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.

Fernando P Hartwig (FP)

Postgraduate Program in Epidemiology, Federal University of Pelotas, Pelotas 96020-220, Brazil; MRC Integrative Epidemiology Unit (IEU), Population Health Sciences, University of Bristol, Bristol BS8 2BN, UK.

Shiow J Lin (SJ)

Division of Statistical Genomics, Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St Louis, MO 63110, USA.

Lihua Wang (L)

Division of Statistical Genomics, Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St Louis, MO 63110, USA.

Tom G Richardson (TG)

MRC Integrative Epidemiology Unit (IEU), Population Health Sciences, University of Bristol, Bristol BS8 2BN, UK.

Jie Yao (J)

Institute for Translational Genomics and Population Sciences, LABioMed and Department of Pediatrics, at Harbor-UCLA Medical Center, Torrance, CA 90502, USA.

Eliana P Fernandez (EP)

Department of Epidemiology, Erasmus University Medical Center, Rotterdam 3015 CE, the Netherlands.

Mohsen Ghanbari (M)

Department of Epidemiology, Erasmus University Medical Center, Rotterdam 3015 CE, the Netherlands.

Mary K Wojczynski (MK)

Division of Statistical Genomics, Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St Louis, MO 63110, USA.

Wen-Jane Lee (WJ)

Department of Medical Research, Taichung Veterans General Hospital, Taichung 407, Taiwan; Department of Social Work, Tunghai University, Taichung 407, Taiwan.

Maria Argos (M)

Department of Epidemiology and Biostatistics, University of Illinois at Chicago, Chicago, IL 60612, USA.

Sebastian M Armasu (SM)

Division of Biomedical Statistics and Informatics, Department of Health Sciences Research, Mayo Clinic, Rochester, MN 55905, USA.

Ruteja A Barve (RA)

Department of Genetics, Washington University School of Medicine, St Louis, MO 63110, USA.

Kathleen A Ryan (KA)

School of Medicine, Division of Endocrinology, Diabetes and Nutrition, and Program for Personalized and Genomic Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Ping An (P)

Division of Statistical Genomics, Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St Louis, MO 63110, USA.

Thomas J Baranski (TJ)

Division of Endocrinology, Metabolism and Lipid Research, Washington University School of Medicine, St. Louis, MO 63110, USA.

Suzette J Bielinski (SJ)

Department of Health Sciences Research, Mayo Clinic, Rochester, MN 55905, USA.

Donald W Bowden (DW)

Center for Diabetes Research, Wake Forest School of Medicine, Cincinnati, OH 45206, USA.

Ulrich Broeckel (U)

Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA.

Kaare Christensen (K)

The Danish Aging Research Center, University of Southern Denmark, Odense 5000, Denmark.

Audrey Y Chu (AY)

Division of Preventive Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA.

Janie Corley (J)

Centre for Cognitive Ageing and Cognitive Epidemiology, University of Edinburgh, Department of Psychology, University of Edinburgh, Edinburgh EH8 9JZ, UK.

Simon R Cox (SR)

Centre for Cognitive Ageing and Cognitive Epidemiology, University of Edinburgh, Department of Psychology, University of Edinburgh, Edinburgh EH8 9JZ, UK.

Andre G Uitterlinden (AG)

Department of Internal Medicine, Erasmus Medical Center, 3000 CA Rotterdam, the Netherlands.

Fernando Rivadeneira (F)

Department of Internal Medicine, Erasmus Medical Center, 3000 CA Rotterdam, the Netherlands.

Cheryl D Cropp (CD)

Samford University McWhorter School of Pharmacy, Birmingham, Alabama, Translational Genomics Research Institute (TGen), Phoenix, AZ 35229, USA.

E Warwick Daw (EW)

Division of Statistical Genomics, Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St Louis, MO 63110, USA.

Diana van Heemst (D)

Department of Internal Medicine, Section of Gerontology and Geriatrics, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.

Lisa de Las Fuentes (L)

Cardiovascular Division, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA.

He Gao (H)

Department of Biostatistics and Epidemiology, MRC-PHE Centre for Environment and Health, School of Public Health, Imperial College London, London W2 1PG, UK.

Ioanna Tzoulaki (I)

Department of Biostatistics and Epidemiology, MRC-PHE Centre for Environment and Health, School of Public Health, Imperial College London, London W2 1PG, UK; Department of Hygiene and Epidemiology, University of Ioannina, Ioannina 45110, Greece.

Tarunveer S Ahluwalia (TS)

Steno Diabetes Center Copenhagen, Copenhagen 2820, Denmark.

Renée de Mutsert (R)

Department of Clinical Epidemiology, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.

Leslie S Emery (LS)

Department of Biostatistics, University of Washington, Seattle, WA 98195, USA.

A Mesut Erzurumluoglu (AM)

Department of Health Sciences, University of Leicester, Leicester LE1 7RH, UK.

James A Perry (JA)

School of Medicine, Division of Endocrinology, Diabetes and Nutrition, and Program for Personalized and Genomic Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Mao Fu (M)

School of Medicine, Division of Endocrinology, Diabetes and Nutrition, and Program for Personalized and Genomic Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Nita G Forouhi (NG)

MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine, Cambridge CB2 0QQ, UK.

Zhenglong Gu (Z)

Division of Nutritional Sciences, Cornell University, Ithaca, NY 14853, USA.

Yang Hai (Y)

Institute for Translational Genomics and Population Sciences, LABioMed and Department of Pediatrics, at Harbor-UCLA Medical Center, Torrance, CA 90502, USA.

Sarah E Harris (SE)

Centre for Cognitive Ageing and Cognitive Epidemiology, University of Edinburgh, Centre for Genomic and Experimental Medicine, Medical Genetics Section, University of Edinburgh, Edinburgh EH4 2XU, UK.

Gibran Hemani (G)

MRC Integrative Epidemiology Unit (IEU), Population Health Sciences, University of Bristol, Bristol BS8 2BN, UK.

Steven C Hunt (SC)

Department of Internal Medicine, University of Utah, Salt Lake City, UT 84132, USA; Department of Genetic Medicine, Weill Cornell Medicine, PO Box 24144, Doha, Qatar.

Marguerite R Irvin (MR)

Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Anna E Jonsson (AE)

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200, Denmark.

Anne E Justice (AE)

Department of Epidemiology, University of North Carolina, Chapel Hill, NC 27516, USA; Biomedical and Translational Informatics, Geisinger Health, Danville, PA 17822, USA.

Nicola D Kerrison (ND)

MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine, Cambridge CB2 0QQ, UK.

Nicholas B Larson (NB)

Department of Health Sciences Research, Mayo Clinic, Rochester, MN 55905, USA.

Keng-Hung Lin (KH)

Department of Ophthalmology, Taichung Veterans General Hospital, Taichung 407, Taiwan.

Latisha D Love-Gregory (LD)

Genomics & Pathology Services, Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO 63110, USA.

Rasika A Mathias (RA)

GeneSTAR Research Program, Division of General Internal Medicine, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA; GeneSTAR Research Program, Divisions of Allergy and Clinical Immunology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Joseph H Lee (JH)

Taub Institute for Research on Alzheimer disease and the Aging Brain, Columbia University Medical Center, New York, NY 10032, USA.

Matthias Nauck (M)

Institute of Clinical Chemistry and Laboratory Medicine, University Medicine Greifswald, Greifswald 17475, Germany.

Raymond Noordam (R)

Department of Internal Medicine, Section of Gerontology and Geriatrics, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.

Ken K Ong (KK)

MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine, Cambridge CB2 0QQ, UK.

James Pankow (J)

University of Minnesota School of Public Health, Division of Epidemiology and Community Health, Minneapolis, MN 55454, USA.

Amit Patki (A)

Department of Biostatistics, School of Public Health, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Alison Pattie (A)

Centre for Cognitive Ageing and Cognitive Epidemiology, University of Edinburgh, Department of Psychology, University of Edinburgh, Edinburgh EH8 9JZ, UK.

Astrid Petersmann (A)

Institute of Clinical Chemistry and Laboratory Medicine, University Medicine Greifswald, Greifswald 17475, Germany.

Qibin Qi (Q)

Department of Epidemiology & Population Health, Albert Einstein School of Medicine, Bronx, NY 10461, USA.

Rasmus Ribel-Madsen (R)

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200, Denmark; Department of Endocrinology, Diabetes and Metabolism, Rigshospitalet, Copenhagen University Hospital, 2100 Copenhagen, Denmark; The Danish Diabetes Academy, 5000 Odense, Denmark.

Rebecca Rohde (R)

Department of Epidemiology, University of North Carolina, Chapel Hill, NC 27516, USA.

Kevin Sandow (K)

Institute for Translational Genomics and Population Sciences, LABioMed and Department of Pediatrics, at Harbor-UCLA Medical Center, Torrance, CA 90502, USA.

Theresia M Schnurr (TM)

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200, Denmark.

Tamar Sofer (T)

Department of Biostatistics, University of Washington, Seattle, WA 98195, USA; Department of Medicine, Harvard Medical School, Boston, MA 02115, USA.

John M Starr (JM)

Centre for Cognitive Ageing and Cognitive Epidemiology, University of Edinburgh, Department of Psychology, University of Edinburgh, Edinburgh EH8 9JZ, UK; Alzheimer Scotland Dementia Research Centre, University of Edinburgh, Edinburgh EH8 9JZ, UK.

Adele M Taylor (AM)

Centre for Cognitive Ageing and Cognitive Epidemiology, University of Edinburgh, Department of Psychology, University of Edinburgh, Edinburgh EH8 9JZ, UK.

Alexander Teumer (A)

Institute for Community Medicine, University Medicine Greifswald, 17475 Greifswald, Germany.

Nicholas J Timpson (NJ)

MRC Integrative Epidemiology Unit (IEU), Population Health Sciences, University of Bristol, Bristol BS8 2BN, UK.

Hugoline G de Haan (HG)

Department of Clinical Epidemiology, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.

Yujie Wang (Y)

Department of Epidemiology, University of North Carolina, Chapel Hill, NC 27516, USA.

Peter E Weeke (PE)

Department of Cardiology, The Heart Centre, Rigshospitalet, University of Copenhagen, Copenhagen 2100, Denmark.

Christine Williams (C)

Division of Statistical Genomics, Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St Louis, MO 63110, USA.

Hongsheng Wu (H)

Computer Science and Networking, Wentworth Institute of Technology, Boston, MA 02115, USA.

Wei Yang (W)

Genome Technology Access Center, Washington University School of Medicine, St Louis, MO 63110, USA.

Donglin Zeng (D)

Department of Biostatistics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.

Daniel R Witte (DR)

Department of Public Health, Section of Epidemiology, Aarhus University, Denmark, Danish Diabetes Academy, Odense University Hospital, 5000 Odense, Denmark.

Bruce S Weir (BS)

Department of Biostatistics, University of Washington, Seattle, WA 98195, USA.

Nicholas J Wareham (NJ)

MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine, Cambridge CB2 0QQ, UK.

Henrik Vestergaard (H)

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200, Denmark; Steno Diabetes Center Copenhagen, Copenhagen 2820, Denmark.

Stephen T Turner (ST)

Division of Nephrology and Hypertension, Department of Cardiovascular Diseases, Mayo Clinic, Rochester, MN 55902, USA.

Christian Torp-Pedersen (C)

Department of Health Science and Technology, Aalborg University Hospital, Aalborg 9220, Denmark.

Evie Stergiakouli (E)

MRC Integrative Epidemiology Unit (IEU), Population Health Sciences, University of Bristol, Bristol BS8 2BN, UK.

Wayne Huey-Herng Sheu (WH)

Division of Endocrinology and Metabolism, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung 407, Taiwan; Institute of Medical Technology, National Chung-Hsing University, Taichung 402, Taiwan; School of Medicine, National Defense Medical Center, Taipei 114, Taiwan; School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.

Frits R Rosendaal (FR)

Department of Clinical Epidemiology, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands.

M Arfan Ikram (MA)

Department of Epidemiology, Erasmus University Medical Center, Rotterdam 3015 CE, the Netherlands.

Oscar H Franco (OH)

Department of Epidemiology, Erasmus University Medical Center, Rotterdam 3015 CE, the Netherlands; Institute of Social and Preventive Medicine (ISPM), University of Bern, 3012 Bern, Switzerland.

Paul M Ridker (PM)

Division of Preventive Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Harvard Medical School, Boston, MA 02115, USA.

Thomas T Perls (TT)

Department of Medicine, Geriatrics Section, Boston University School of Medicine and Boston Medical Center, Boston, MA 02118, USA.

Oluf Pedersen (O)

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200, Denmark.

Ellen A Nohr (EA)

Research Unit for Gynecology and Obstetrics, Department of Clinical Research, University of Southern Denmark, 5000 Odense, Denmark.

Anne B Newman (AB)

Department of Epidemiology, University of Pittsburgh, Pittsburgh, PA 15261, USA.

Allan Linneberg (A)

Department of Clinical Experimental Research, Rigshospitalet, Copenhagen 2200, Denmark; Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200, Denmark; The Center for Clinical Research and Prevention, Bispebjerg and Frederiksberg Hospital, The Capital Region, Copenhagen 2000, Denmark.

Claudia Langenberg (C)

MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine, Cambridge CB2 0QQ, UK.

Tuomas O Kilpeläinen (TO)

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200, Denmark.

Sharon L R Kardia (SLR)

Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI 48109, USA.

Marit E Jørgensen (ME)

Steno Diabetes Center Copenhagen, Copenhagen 2820, Denmark.

Torben Jørgensen (T)

Research Centre for Prevention and Health, Glostrup Hospital, Glostrup 2600, Denmark; Department of Public Health, Faculty of Health Sciences, University of Copenhagen, Copenhagen 1014, Denmark; Faculty of Medicine, Aalborg University, Aalborg 9100, Denmark.

Thorkild I A Sørensen (TIA)

Novo Nordisk Foundation Center for Basic Metabolic Research (Section of Metabolic Genetics) and Department of Public Health (Section on Epidemiology), Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200N, Denmark.

Georg Homuth (G)

Interfaculty Institute for Genetics and Functional Genomics, University Medicine and University of Greifswald, Greifswald 17475, Germany.

Torben Hansen (T)

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200, Denmark.

Mark O Goodarzi (MO)

Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA.

Ian J Deary (IJ)

Centre for Cognitive Ageing and Cognitive Epidemiology, University of Edinburgh, Department of Psychology, University of Edinburgh, Edinburgh EH8 9JZ, UK.

Cramer Christensen (C)

Department of Internal Medicine, Section of Endocrinology, Vejle Lillebaelt Hospital, 7100 Vejle, Denmark.

Yii-Der Ida Chen (YI)

Institute for Translational Genomics and Population Sciences, LABioMed and Department of Pediatrics, at Harbor-UCLA Medical Center, Torrance, CA 90502, USA.

Aravinda Chakravarti (A)

Center for Complex Disease Genomics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Ivan Brandslund (I)

Department of Clinical Biochemistry, Vejle Hospital, 7100 Vejle, Denmark; Institute of Regional Health Research, University of Southern Denmark, 5000 Odense C, Denmark.

Klaus Bonnelykke (K)

Copenhagen Prospective Studies on Asthma in Childhood, Copenhagen University Hospital, Gentofte & Naestved 2820, Denmark; Health Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.

Kent D Taylor (KD)

Institute for Translational Genomics and Population Sciences, LABioMed and Department of Pediatrics, at Harbor-UCLA Medical Center, Torrance, CA 90502, USA.

James G Wilson (JG)

Department of Physiology and Biophysics, University of Mississippi Medical Center, Jackson, MS 39216, USA.

Santiago Rodriguez (S)

MRC Integrative Epidemiology Unit (IEU), Population Health Sciences, University of Bristol, Bristol BS8 2BN, UK.

Gail Davies (G)

Centre for Cognitive Ageing and Cognitive Epidemiology, University of Edinburgh, Department of Psychology, University of Edinburgh, Edinburgh EH8 9JZ, UK.

Bernardo L Horta (BL)

Postgraduate Program in Epidemiology, Federal University of Pelotas, Pelotas 96020-220, Brazil.

Bharat Thyagarajan (B)

Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455, USA.

D C Rao (DC)

Division of Biostatistics, Washington University School of Medicine, St Louis, MO 63110, USA.

Niels Grarup (N)

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen 2200, Denmark.

Victor G Davila-Roman (VG)

Cardiovascular Division, Department of Medicine, Washington University School of Medicine, St Louis, MO 63110, USA.

Gavin Hudson (G)

Wellcome Trust Centre for Mitochondrial Research, Institute of Neuroscience, Newcastle University, Newcastle upon Tyne NE1 3BZ, UK.

Xiuqing Guo (X)

Institute for Translational Genomics and Population Sciences, LABioMed and Department of Pediatrics, at Harbor-UCLA Medical Center, Torrance, CA 90502, USA.

Donna K Arnett (DK)

University of Kentucky, College of Public Health, Lexington, KY 40508, USA.

Caroline Hayward (C)

MRC Human Genetics Unit, University of Edinburgh, Institute of Genetics and Molecular Medicine, Western General Hospital, Edinburgh EH4 2XU, UK.

Dhananjay Vaidya (D)

GeneSTAR Research Program, Division of General Internal Medicine, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Dennis O Mook-Kanamori (DO)

Department of Clinical Epidemiology, Leiden University Medical Center, Leiden 2333 ZA, the Netherlands; Department of Public Health and Primary Care, Leiden University Medical Center, 2333 ZA Leiden, the Netherlands.

Hemant K Tiwari (HK)

Department of Biostatistics, School of Public Health, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Daniel Levy (D)

The Framingham Heart Study, Framingham, MA, USA; The Population Sciences Branch, NHLBI/NIH, Bethesda, MD 20892, USA.

Ruth J F Loos (RJF)

Charles Bronfman Institute for Personalized Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Genetics of Obesity and Related Traits Program, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

Abbas Dehghan (A)

Department of Biostatistics and Epidemiology, MRC-PHE Centre for Environment and Health, School of Public Health, Imperial College London, London W2 1PG, UK.

Paul Elliott (P)

Department of Biostatistics and Epidemiology, MRC-PHE Centre for Environment and Health, School of Public Health, Imperial College London, London W2 1PG, UK.

Afshan N Malik (AN)

King's College London, Department of Diabetes, School of Life Course, Faculty of Life Sciences and Medicine, London SE1 1NN, UK.

Robert A Scott (RA)

MRC Epidemiology Unit, University of Cambridge School of Clinical Medicine, Cambridge CB2 0QQ, UK.

Diane M Becker (DM)

GeneSTAR Research Program, Division of General Internal Medicine, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Mariza de Andrade (M)

Division of Biomedical Statistics and Informatics, Department of Health Sciences Research, Mayo Clinic, Rochester, MN 55905, USA.

Michael A Province (MA)

Division of Statistical Genomics, Department of Genetics, Center for Genome Sciences and Systems Biology, Washington University School of Medicine, St Louis, MO 63110, USA.

James B Meigs (JB)

Department of Medicine, Harvard Medical School, Boston, MA 02115, USA; Division of General Internal Medicine, Massachusetts General Hospital, Boston 02114, MA, USA; Program in Medical and Population Genetics, Broad Institute, Boston, MA 02142, USA.

Jerome I Rotter (JI)

Institute for Translational Genomics and Population Sciences, LABioMed and Department of Pediatrics, at Harbor-UCLA Medical Center, Torrance, CA 90502, USA.

Kari E North (KE)

Department of Epidemiology, University of North Carolina, Chapel Hill, NC 27516, USA. Electronic address: kari_north@unc.edu.

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