Dissociating effect of salivary gland extract from Ixodes ricinus on human fibroblasts: Potential impact on Borrelia transmission.
Borrelia
Feeding pool
Fibroblast
Histone H4
Ixodes
Mass spectrometry
Tick saliva
Journal
Ticks and tick-borne diseases
ISSN: 1877-9603
Titre abrégé: Ticks Tick Borne Dis
Pays: Netherlands
ID NLM: 101522599
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
15
01
2018
revised:
19
12
2018
accepted:
21
12
2018
pubmed:
1
1
2019
medline:
5
4
2019
entrez:
1
1
2019
Statut:
ppublish
Résumé
Understanding the mechanism of pathogen transmission is essential for the development of strategies to reduce arthropod-borne diseases. The pharmaco- and immunomodulatory properties of insect and acarine saliva play an essential role in the efficiency of pathogen transmission. The skin as the site where arthropod saliva and pathogens are inoculated - represents the key interface in vector-borne diseases. We identified tick molecules potentially involved in pathogen transmission, using micro-HPLC and mass spectrometry, followed by in vitro assays on human skin cells. Histone H4 isolated from Ixodes ricinus salivary gland extract was identified as a molecule with a dissociating effect on human primary fibroblasts. This histone might be involved in the formation of the feeding pool formed around the tick mouthparts and responsible of tissue necrosis in the vertebrate host. Thanks to its selective antimicrobial activity, it may also sterilize the feeding pool and facilitate transmission of pathogens such as Borrelia burgdorferi sensu lato.
Identifiants
pubmed: 30595500
pii: S1877-959X(18)30014-1
doi: 10.1016/j.ttbdis.2018.12.005
pii:
doi:
Substances chimiques
Histones
0
Tissue Extracts
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
433-441Informations de copyright
Copyright © 2018 Elsevier GmbH. All rights reserved.