Phagolysosomal activity of macrophages in Nile tilapia (Oreochromis niloticus) infected in vitro by Aeromonas hydrophila: Infection and immunotherapy.
Acid Phosphatase
/ metabolism
Aeromonas hydrophila
/ physiology
Animals
Cichlids
/ immunology
Fish Diseases
/ immunology
Gram-Negative Bacterial Infections
/ immunology
Immunoglobulins
/ immunology
In Vitro Techniques
Macrophages
/ immunology
Nitric Oxide
/ metabolism
Phagocytosis
Reactive Oxygen Species
/ metabolism
Chronic inflammation
Immunoglobulin Y
Phagocytosis
Teleost
Journal
Fish & shellfish immunology
ISSN: 1095-9947
Titre abrégé: Fish Shellfish Immunol
Pays: England
ID NLM: 9505220
Informations de publication
Date de publication:
Apr 2019
Apr 2019
Historique:
received:
16
11
2018
revised:
22
12
2018
accepted:
28
12
2018
pubmed:
2
1
2019
medline:
29
8
2019
entrez:
2
1
2019
Statut:
ppublish
Résumé
The biochemical mechanisms involved in phagocytosis and the intracellular survival of Aeromonas hydrophila (Ah) in host macrophages (MΦs) are complex processes that affect infection success or failure. Thus, in the present study, we described the in vitro infection of Nile tilapia MΦs by a homologous bacterium and tested the effects of anti-A. hydrophila immunoglobulin Y (IgY) on the phagolysosomal activity and intracellular survival of the pathogen. The anti-Ah IgY modulated lysosomal acid phosphatase (LAP) activity as well as the production of reactive oxygen intermediates (ROIs) and nitric oxide (NO), thereby potentiating phagocytosis and the elimination of Ah. Thus, we assume that the specific IgY had a beneficial effect on infection control and postulated the use of the Nile tilapia MΦs as an important in vitro experimental model for the functional and therapeutic study of Ah infection.
Identifiants
pubmed: 30599256
pii: S1050-4648(18)30880-5
doi: 10.1016/j.fsi.2018.12.074
pii:
doi:
Substances chimiques
IgY
0
Immunoglobulins
0
Reactive Oxygen Species
0
Nitric Oxide
31C4KY9ESH
Acid Phosphatase
EC 3.1.3.2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
51-61Informations de copyright
Copyright © 2018 Elsevier Ltd. All rights reserved.