The Pathogen-Host Interface in Three Dimensions: Correlative FIB/SEM Applications.
Brucella
FIB/SEM
Salmonella
Shigella
correlative microscopy
volume electron microscopy
Journal
Trends in microbiology
ISSN: 1878-4380
Titre abrégé: Trends Microbiol
Pays: England
ID NLM: 9310916
Informations de publication
Date de publication:
05 2019
05 2019
Historique:
received:
27
09
2018
revised:
27
11
2018
accepted:
30
11
2018
pubmed:
3
1
2019
medline:
1
7
2020
entrez:
3
1
2019
Statut:
ppublish
Résumé
Pathogens survive and propagate within host cells through a wide array of complex interactions. Tracking the molecular and cellular events by multidimensional fluorescence microscopy has been a widespread tool for research on intracellular pathogens. Through major advancements in 3D electron microscopy, intracellular pathogens can also be visualized in their cellular environment to an unprecedented level of detail within large volumes. Recently, multidimensional fluorescence microscopy has been correlated with volume electron microscopy, combining molecular and functional information with the overall ultrastructure of infection events. In this review, we provide a short introduction to correlative focused ion beam/scanning electron microscopy (c-FIB/SEM) tomography and illustrate its utility for intracellular pathogen research through a series of studies on Shigella, Salmonella, and Brucella cellular invasion. We conclude by discussing current limitations of and prospects for this approach.
Identifiants
pubmed: 30600140
pii: S0966-842X(18)30273-7
doi: 10.1016/j.tim.2018.11.011
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
426-439Informations de copyright
Copyright © 2018 Elsevier Ltd. All rights reserved.