Hypoxia-Inducible Factor (HIF)-1α Promotes Inflammation and Injury Following Aspiration-Induced Lung Injury in Mice.


Journal

Shock (Augusta, Ga.)
ISSN: 1540-0514
Titre abrégé: Shock
Pays: United States
ID NLM: 9421564

Informations de publication

Date de publication:
12 2019
Historique:
pubmed: 3 1 2019
medline: 20 8 2020
entrez: 3 1 2019
Statut: ppublish

Résumé

Acid aspiration-induced lung injury is a common disease in the intensive care unit (ICU) and acute respiratory distress syndrome (ARDS). Hypoxia-inducible factor (HIF)-1α is a major transcription factor responsible for regulating the cellular response to changes in oxygen tension. A clear understanding of the function of HIF-1α in lung inflammatory response is currently lacking. Here, we sought to determine the role of HIF-1α in type 2 alveolar epithelial cells (AEC) in the generation of the acute inflammatory response following gastric aspiration (GA). GA led to profound hypoxia at very early time points following GA. This correlated to a robust increase in HIF-1α, tissue albumin and pro-inflammatory mediators following GA in AECs. The extent of lung injury and the release of pro/anti-inflammatory cytokines were significantly reduced in HIF-1α (-/-) mice. Finally, we report that HIF-1α upregulation of the acute inflammatory response is dependent on NF-κB following GA.

Identifiants

pubmed: 30601332
doi: 10.1097/SHK.0000000000001312
pmc: PMC6591116
mid: NIHMS1517045
doi:

Substances chimiques

Hif1a protein, mouse 0
Hypoxia-Inducible Factor 1, alpha Subunit 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

612-621

Subventions

Organisme : NCI NIH HHS
ID : R01 CA148828
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM111305
Pays : United States

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Auteurs

Madathilparambil V Suresh (MV)

Department of Surgery, University of Michigan, Ann Arbor, Michigan.

Sanjay Balijepalli (S)

Department of Surgery, University of Michigan, Ann Arbor, Michigan.

Boya Zhang (B)

Department of Surgery, University of Michigan, Ann Arbor, Michigan.

Vikas Vikram Singh (VV)

Department of Surgery, University of Michigan, Ann Arbor, Michigan.

Samantha Swamy (S)

Department of Surgery, University of Michigan, Ann Arbor, Michigan.

Sreehari Panicker (S)

Department of Surgery, University of Michigan, Ann Arbor, Michigan.

Vladislov A Dolgachev (VA)

Department of Surgery, University of Michigan, Ann Arbor, Michigan.

Chitra Subramanian (C)

Department of Surgery, University of Michigan, Ann Arbor, Michigan.

Sadeesh K Ramakrishnan (SK)

Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan.

Bivin Thomas (B)

Department of Surgery, University of Michigan, Ann Arbor, Michigan.

Tejeshwar C Rao (TC)

Department of Cell, Development and Integrative Biology, University of Alabama at Birmingham, Birmingham, Alabama.

Matthew J Delano (MJ)

Department of Surgery, University of Michigan, Ann Arbor, Michigan.

David Machado-Aranda (D)

Department of Surgery, University of Michigan, Ann Arbor, Michigan.

Yatrik M Shah (YM)

Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan.

Krishnan Raghavendran (K)

Department of Surgery, University of Michigan, Ann Arbor, Michigan.

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