Generating Vegfr3 reporter transgenic mouse expressing membrane-tagged Venus for visualization of VEGFR3 expression in vascular and lymphatic endothelial cells.
Animals
Bacterial Proteins
/ genetics
Blood Vessels
/ metabolism
Cell Line, Tumor
Cell Membrane
/ metabolism
Embryo, Mammalian
/ blood supply
Endothelial Cells
/ metabolism
Female
GAP-43 Protein
/ genetics
Gene Expression
Luminescent Proteins
/ genetics
Lymphatic Vessels
/ cytology
Mice, Inbred C57BL
Mice, Inbred ICR
Mice, Transgenic
Microscopy, Confocal
Neoplasms, Experimental
/ blood supply
Neovascularization, Pathologic
/ genetics
Vascular Endothelial Growth Factor Receptor-3
/ genetics
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
31
01
2018
accepted:
08
11
2018
entrez:
3
1
2019
pubmed:
3
1
2019
medline:
1
10
2019
Statut:
epublish
Résumé
Vascular endothelial growth factor receptor 3 (Vegfr3) has been widely used as a marker for lymphatic and vascular endothelial cells during mouse embryonic development and in adult mouse, making it valuable for studying angiogenesis and lymphangiogenesis under normal and pathological conditions. Here, we report the generation of a novel transgenic (Tg) mouse that expresses a membrane-localized fluorescent reporter protein, Gap43-Venus, under the control of the Vegfr3 regulatory sequence. Vegfr3-Gap43-Venus BAC Tg recapitulated endogenous Vegfr3 expression in vascular and lymphatic endothelial cells during embryonic development and tumor development. Thus, this Tg mouse line contributes a valuable model to study angiogenesis and lymphangiogenesis in physiological and pathological contexts.
Identifiants
pubmed: 30601868
doi: 10.1371/journal.pone.0210060
pii: PONE-D-18-03286
pmc: PMC6314617
doi:
Substances chimiques
Bacterial Proteins
0
GAP-43 Protein
0
Luminescent Proteins
0
yellow fluorescent protein, Bacteria
0
Vascular Endothelial Growth Factor Receptor-3
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0210060Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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