Synthesis and biological activity of a potent optically pure autoinducer-2 quorum sensing agonist.


Journal

Bioorganic chemistry
ISSN: 1090-2120
Titre abrégé: Bioorg Chem
Pays: United States
ID NLM: 1303703

Informations de publication

Date de publication:
04 2019
Historique:
received: 22 06 2018
revised: 17 11 2018
accepted: 16 12 2018
pubmed: 3 1 2019
medline: 12 2 2020
entrez: 3 1 2019
Statut: ppublish

Résumé

Quorum sensing (QS) regulates population-dependent bacterial behaviours, such as toxin production, biofilm formation and virulence. Autoinducer-2 (AI-2) is to date the only signalling molecule known to foster inter-species bacterial communication across distantly related bacterial species. In this work, the synthesis of pure enantiomers of C4-propoxy-HPD and C4-ethoxy-HPD, known AI-2 analogues, has been developed. The optimised synthesis is efficient, reproducible and short. The (4S) enantiomer of C4-propoxy-HPD was the most active compound being approximately twice as efficient as (4S)-DPD and ten-times more potent than the (4R) enantiomer. Additionally, the specificity of this analogue to bacteria with LuxP receptors makes it a good candidate for clinical applications, because it is not susceptible to scavenging by LsrB-containing bacteria that degrade the natural AI-2. All in all, this study provides a new brief and effective synthesis of isomerically pure analogues for QS modulation that include the most active AI-2 agonist described so far.

Identifiants

pubmed: 30602129
pii: S0045-2068(18)30616-3
doi: 10.1016/j.bioorg.2018.12.022
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0
Bacterial Proteins 0
Carrier Proteins 0
Escherichia coli Proteins 0
LsrB protein, E coli 0
LuxP protein, Vibrio 0
Pentanones 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

75-81

Informations de copyright

Copyright © 2018 Elsevier Inc. All rights reserved.

Auteurs

Osvaldo S Ascenso (OS)

Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2780-901 Oeiras, Portugal.

Inês M Torcato (IM)

Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2780-901 Oeiras, Portugal; Instituto Gulbenkian de Ciência, 2781-901 Oeiras, Portugal.

Ana Sofia Miguel (AS)

Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2780-901 Oeiras, Portugal.

João C Marques (JC)

Rowland Institute at Harvard, 100 Edwin H. Land Boulevard, Cambridge, MA 02142, USA(2).

Karina B Xavier (KB)

Instituto Gulbenkian de Ciência, 2781-901 Oeiras, Portugal.

M Rita Ventura (MR)

Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2780-901 Oeiras, Portugal. Electronic address: rventura@itqb.unl.pt.

Christopher D Maycock (CD)

Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2780-901 Oeiras, Portugal; Faculdade de Ciências da Universidade de Lisboa, Departamento de Química e Bioquímica, 1749-016 Lisboa, Portugal. Electronic address: maycock@itqb.unl.pt.

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Classifications MeSH