Clonal anergy of CD117
Animals
Antibodies, Viral
/ blood
Avian Leukosis
/ congenital
Avian Leukosis Virus
/ immunology
B-Lymphocyte Subsets
/ chemistry
Bursa of Fabricius
/ pathology
Cell Differentiation
Cell Proliferation
Chickens
Clonal Anergy
Flow Cytometry
Immunoglobulin G
/ blood
Immunoglobulin M
/ blood
Poultry Diseases
/ congenital
Proto-Oncogene Proteins c-kit
/ analysis
Stem Cells
/ chemistry
Avian leukosis virus subgroup J
B cell anergy
B cell progenitor
Congenital infection
Immunological tolerance
Journal
Retrovirology
ISSN: 1742-4690
Titre abrégé: Retrovirology
Pays: England
ID NLM: 101216893
Informations de publication
Date de publication:
03 01 2019
03 01 2019
Historique:
received:
09
09
2018
accepted:
24
12
2018
entrez:
4
1
2019
pubmed:
4
1
2019
medline:
28
3
2019
Statut:
epublish
Résumé
The pathogenesis of immunological tolerance caused by avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, is largely unknown. In this study, the development, differentiation, and immunological capability of B cells and their progenitors infected with ALV-J were studied both morphologically and functionally by using a model of ALV-J congenital infection. Compared with posthatch infection, congenital infection of ALV-J resulted in severe immunological tolerance, which was identified as the absence of detectable specific antivirus antibodies. In congenitally infected chickens, immune organs, particularly the bursa of Fabricius, were poorly developed. Moreover, IgM-and IgG-positive cells and total immunoglobulin levels were significantly decreased in these chickens. Large numbers of bursa follicles with no differentiation into cortex and medulla indicated that B cell development was arrested at the early stage. Flow cytometry analysis further confirmed that ALV-J blocked the differentiation of CD117 These findings suggested that the anergy of B cells in congenitally infected chickens is caused by the developmental arrest and dysfunction of B cell progenitors, which is an important factor for the immunological tolerance induced by ALV-J.
Sections du résumé
BACKGROUND
The pathogenesis of immunological tolerance caused by avian leukosis virus subgroup J (ALV-J), an oncogenic retrovirus, is largely unknown.
RESULTS
In this study, the development, differentiation, and immunological capability of B cells and their progenitors infected with ALV-J were studied both morphologically and functionally by using a model of ALV-J congenital infection. Compared with posthatch infection, congenital infection of ALV-J resulted in severe immunological tolerance, which was identified as the absence of detectable specific antivirus antibodies. In congenitally infected chickens, immune organs, particularly the bursa of Fabricius, were poorly developed. Moreover, IgM-and IgG-positive cells and total immunoglobulin levels were significantly decreased in these chickens. Large numbers of bursa follicles with no differentiation into cortex and medulla indicated that B cell development was arrested at the early stage. Flow cytometry analysis further confirmed that ALV-J blocked the differentiation of CD117
CONCLUSIONS
These findings suggested that the anergy of B cells in congenitally infected chickens is caused by the developmental arrest and dysfunction of B cell progenitors, which is an important factor for the immunological tolerance induced by ALV-J.
Identifiants
pubmed: 30602379
doi: 10.1186/s12977-018-0463-9
pii: 10.1186/s12977-018-0463-9
pmc: PMC6317241
doi:
Substances chimiques
Antibodies, Viral
0
Immunoglobulin G
0
Immunoglobulin M
0
Proto-Oncogene Proteins c-kit
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1Subventions
Organisme : National Natural Science Foundation of China
ID : 31672521
Pays : International
Organisme : China-UK Partnership on Global Food Security: Combating Avian Leukosis Virus Subgroup J for Sustainable Poultry Production
ID : 31761133002
Pays : International
Organisme : China-UK Partnership on Global Food Security: Combating Avian Leukosis Virus Subgroup J for Sustainable Poultry Production
ID : BB/R012865/1
Pays : International
Organisme : Shandong Modern Agricultural Technology & Industry System
ID : SDAIT-11-04
Pays : International
Organisme : Fund of Shandong "Double Tops" Program
ID : 2017
Pays : International
Références
Immunol Rev. 2010 Sep;237(1):249-63
pubmed: 20727040
Viruses. 2018 May 18;10(5):
pubmed: 29783672
J Vet Med Sci. 2010 Aug;72(8):1027-33
pubmed: 20467208
In Vitro Cell Dev Biol Anim. 2018 Jan;54(1):41-51
pubmed: 29197030
J Immunol. 2000 May 15;164(10):5041-8
pubmed: 10799859
Poult Sci. 1998 Aug;77(8):1204-12
pubmed: 9706091
Res Vet Sci. 1997 Jul-Aug;63(1):81-3
pubmed: 9368962
Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10806-11
pubmed: 10485907
Virus Res. 2014 Aug 8;188:8-14
pubmed: 24680656
Front Immunol. 2016 Dec 21;7:624
pubmed: 28066434
Avian Dis. 2004 Jan-Mar;48(1):61-7
pubmed: 15077798
Front Immunol. 2018 Mar 14;9:462
pubmed: 29593723
Nat Rev Immunol. 2012 Sep;12(9):636-48
pubmed: 22918466
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1602-6
pubmed: 6966401
Avian Pathol. 2015;44(1):43-9
pubmed: 25484188
Aust Vet J. 2004 Nov;82(11):701-6
pubmed: 15977617
J Gen Virol. 2017 Dec;98(12):2918-2930
pubmed: 29154745
Virology. 1962 May;17:143-56
pubmed: 14494904
J Virol. 2001 Feb;75(4):2029-32
pubmed: 11160706
Oncogene. 2001 May 31;20(25):3226-34
pubmed: 11423972
J Exp Med. 2009 Jan 16;206(1):139-51
pubmed: 19103878
J Vet Sci. 2003 Dec;4(3):245-55
pubmed: 14685030
Immunity. 2006 Dec;25(6):953-62
pubmed: 17174121
Leukemia. 1992 Nov;6(11):1167-76
pubmed: 1331625
Curr Opin Immunol. 2017 Aug;47:26-34
pubmed: 28728075
Mol Immunol. 2015 Mar;64(1):106-11
pubmed: 25438822
Avian Pathol. 2018 Feb;47(1):83-89
pubmed: 28859493
Nature. 1953 Oct 3;172(4379):603-6
pubmed: 13099277
Curr Top Microbiol Immunol. 1986;132:209-14
pubmed: 3024913
Adv Immunol. 1994;56:467-84
pubmed: 8073951
Science. 1987 Nov 20;238(4830):1094-8
pubmed: 3317827
J Vet Sci. 2011 Sep;12(3):235-41
pubmed: 21897096
Retrovirology. 2018 Jul 3;15(1):45
pubmed: 29970099
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3418-22
pubmed: 6267589
Avian Dis. 2004 Dec;48(4):921-7
pubmed: 15666876
Nat Rev Immunol. 2007 Aug;7(8):633-43
pubmed: 17641666
Eur J Immunol. 1987 Apr;17(4):503-7
pubmed: 3569406
Nature. 1984 Sep 6-11;311(5981):69-71
pubmed: 6433205
Comp Immunol Microbiol Infect Dis. 1979;2(4):519-29
pubmed: 232865
Semin Immunopathol. 2017 Nov;39(6):643-652
pubmed: 28685270
Avian Dis. 2003 Oct-Dec;47(4):1321-30
pubmed: 14708978
Immunol Today. 1985 Jul;6(7):223-7
pubmed: 25290185
Eur J Immunol. 1994 Feb;24(2):458-63
pubmed: 8299695
Avian Dis. 1999 Jul-Sep;43(3):391-400
pubmed: 10494407
Oncotarget. 2017 Oct 13;8(59):99889-99900
pubmed: 29245947
J Gen Virol. 1999 Jan;80 ( Pt 1):261-268
pubmed: 9934710
Nat Rev Immunol. 2017 Feb;17(2):83-96
pubmed: 28044057
Sci Transl Med. 2016 Apr 27;8(336):336ra62
pubmed: 27122615
Vet Pathol. 1997 Mar;34(2):127-37
pubmed: 9066079
J Virol. 2000 Feb;74(3):1296-306
pubmed: 10627540
Vet Immunol Immunopathol. 2016 Sep;177:42-7
pubmed: 27436443
Avian Pathol. 2012;41(1):11-9
pubmed: 22845317