YB-1, an abundant core mRNA-binding protein, has the capacity to form an RNA nucleoprotein filament: a structural analysis.
Amino Acid Sequence
/ genetics
Cytoskeleton
/ genetics
Escherichia coli
/ genetics
Humans
Nucleoproteins
/ chemistry
Protein Binding
/ genetics
Protein Biosynthesis
/ genetics
Protein Folding
RNA, Messenger
/ chemistry
RNA-Binding Proteins
/ genetics
Ribosomes
/ chemistry
Y-Box-Binding Protein 1
/ chemistry
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
08 04 2019
08 04 2019
Historique:
accepted:
20
12
2018
revised:
17
12
2018
received:
26
09
2018
pubmed:
4
1
2019
medline:
12
10
2019
entrez:
4
1
2019
Statut:
ppublish
Résumé
The structural rearrangements accompanying mRNA during translation in mammalian cells remain poorly understood. Here, we discovered that YB-1 (YBX1), a major partner of mRNAs in the cytoplasm, forms a linear nucleoprotein filament with mRNA, when part of the YB-1 unstructured C-terminus has been truncated. YB-1 possesses a cold-shock domain (CSD), a remnant of bacterial cold shock proteins that have the ability to stimulate translation under the low temperatures through an RNA chaperone activity. The structure of the nucleoprotein filament indicates that the CSD of YB-1 preserved its chaperone activity also in eukaryotes and shows that mRNA is channeled between consecutive CSDs. The energy benefit needed for the formation of stable nucleoprotein filament relies on an electrostatic zipper mediated by positively charged amino acid residues in the YB-1 C-terminus. Thus, YB-1 displays a structural plasticity to unfold structured mRNAs into extended linear filaments. We anticipate that our findings will shed the light on the scanning of mRNAs by ribosomes during the initiation and elongation steps of mRNA translation.
Identifiants
pubmed: 30605522
pii: 5272362
doi: 10.1093/nar/gky1303
pmc: PMC6451097
doi:
Substances chimiques
Nucleoproteins
0
RNA, Messenger
0
RNA-Binding Proteins
0
Y-Box-Binding Protein 1
0
YBX1 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3127-3141Informations de copyright
© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.
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