Injectable hydrogels from enzyme-catalyzed crosslinking as BMSCs-laden scaffold for bone repair and regeneration.
BMSCs-laden scaffold
Enzyme-catalyzed crosslinking
Injectable hydrogels
Osteogenic differentiation
Tissue engineering
Journal
Materials science & engineering. C, Materials for biological applications
ISSN: 1873-0191
Titre abrégé: Mater Sci Eng C Mater Biol Appl
Pays: Netherlands
ID NLM: 101484109
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
08
03
2018
revised:
30
10
2018
accepted:
05
12
2018
entrez:
5
1
2019
pubmed:
5
1
2019
medline:
13
3
2019
Statut:
ppublish
Résumé
Bone-marrow-derived mesenchymal stem cells possess great potential for tissue engineering and regenerative medicine. In the work, an injectable BMSCs-laden hydrogel system was formed by enzyme-catalyzed crosslinking of hyaluronic acid-tyramine and chondroitin sulfate-tyramine in the presence of hydrogen peroxide and horseradish peroxidase, which was used as a 3D scaffold to explore the behavior of the mesenchymal stem cells. Afterward, the gelation rate, mechanical properties, as well as the degradation process of the scaffold were well characterized and optimized. Furthermore, bone morphogenetic protein-2 was encapsulated in the scaffold, which was used to improve the osteogenic properties. The results illustrated that such a BMSCs-laden hydrogel not only offered a proper microenvironment for the adhesion, proliferation and differentiation of mesenchymal stem cells in vitro, but also promoted bone regeneration in vivo. Therefore, this injectable BMSCs-laden hydrogel may serve as an efficient 3D scaffold for bone repair and regeneration.
Identifiants
pubmed: 30606598
pii: S0928-4931(18)30711-2
doi: 10.1016/j.msec.2018.12.014
pii:
doi:
Substances chimiques
Hydrogels
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
841-849Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.