Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell cultures.
cell culture
cervical cancer
ectocervix
endocervix
human papillomavirus (HPV)
organotypic culture
transformation zone (TZ)
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
06 2019
06 2019
Historique:
received:
10
08
2018
accepted:
03
12
2018
pubmed:
5
1
2019
medline:
9
4
2020
entrez:
5
1
2019
Statut:
ppublish
Résumé
Cervical cancer is a major public health problem and research using cell culture models has improved understanding of this disease. The human cervix contains three anatomic regions; ectocervix with stratified squamous epithelium, endocervix with secretory epithelium, and transformation zone (TZ) with metaplastic cells. Most cervical cancers originate within the TZ. However, little is known about the biology of TZ cells or why they are highly susceptible to carcinogenesis. The goal of this study was to develop and optimize methods to compare growth and differentiation of cells cultured from ectocervix, TZ or endocervix. We examined the effects of different serum-free media on cell attachment, cell growth and differentiation, and cell population doublings in monolayer culture. We also optimized conditions for organotypic culture of cervical epithelial cells using collagen rafts with human cervical stromal cells. Finally, we present a step-by-step protocol for culturing cells from each region of human cervix.
Identifiants
pubmed: 30609028
doi: 10.1002/jcp.28049
pmc: PMC6395493
mid: NIHMS1003958
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7683-7694Subventions
Organisme : NCI NIH HHS
ID : R15 CA173703
Pays : United States
Informations de copyright
© 2019 Wiley Periodicals, Inc.
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