Single cell analyses reveal specific distribution of anti-bacterial molecule Perforin-2 in human skin and its modulation by wounding and Staphylococcus aureus infection.
Animals
Cell Membrane
/ metabolism
Endothelial Cells
/ immunology
Fibroblasts
/ metabolism
HEK293 Cells
Humans
Immunophenotyping
In Situ Hybridization, Fluorescence
Intraepithelial Lymphocytes
/ immunology
Keratinocytes
/ immunology
Leukocyte Common Antigens
/ metabolism
Mice
Pore Forming Cytotoxic Proteins
/ metabolism
Single-Cell Analysis
/ methods
Skin
/ metabolism
Staphylococcal Infections
/ metabolism
Staphylococcus aureus
Wound Healing
Perforin-2
fish-flow RNA assay
intracellular bacteria
skin
wound
Journal
Experimental dermatology
ISSN: 1600-0625
Titre abrégé: Exp Dermatol
Pays: Denmark
ID NLM: 9301549
Informations de publication
Date de publication:
03 2019
03 2019
Historique:
received:
04
05
2018
revised:
03
12
2018
accepted:
02
01
2019
pubmed:
5
1
2019
medline:
30
7
2020
entrez:
5
1
2019
Statut:
ppublish
Résumé
Perforin-2 (P-2) is a recently described antimicrobial protein with unique properties to kill intracellular bacteria. We investigated P-2 expression pattern and cellular distribution in human skin and its importance in restoration of barrier function during wound healing process and infection with the common wound pathogen Staphylococcus aureus. We describe a novel approach for the measurement of P-2 mRNA within individual skin cells using an amplified fluorescence in situ hybridization (FISH) technique. The unique aspect of this approach is simultaneous detection of P-2 mRNA in combination with immune-phenotyping for cell surface proteins using fluorochrome-conjugated antibodies. We detected P-2 transcript in both hematopoietic (CD45
Identifiants
pubmed: 30609079
doi: 10.1111/exd.13870
pmc: PMC7461719
mid: NIHMS1005243
doi:
Substances chimiques
Pore Forming Cytotoxic Proteins
0
perforin 2
0
Leukocyte Common Antigens
EC 3.1.3.48
PTPRC protein, human
EC 3.1.3.48
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
225-232Subventions
Organisme : NINR NIH HHS
ID : R01 NR015649
Pays : United States
Informations de copyright
© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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