In vitro selection of CD70 binding aptamer and its application in a biosensor design for sensitive detection of SKOV-3 ovarian cells.

Aptamers, Nucleotide / genetics Base Sequence Biosensing Techniques / methods CD27 Ligand / metabolism Cell Line, Tumor Female Humans Ovarian Neoplasms / pathology
Aptasensor Biomarker CD70 Cancer DNA aptamer SELEX

Journal

Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R

Informations de publication

Date de publication:
01 Mar 2019
Historique:
received: 20 08 2018
revised: 16 10 2018
accepted: 18 10 2018
entrez: 6 1 2019
pubmed: 6 1 2019
medline: 13 2 2019
Statut: ppublish

Résumé

Single-stranded DNA aptamers recognizing CD70 molecule overexpressed in some tumor cell lines was isolated by means of systematic evolution of ligands by exponential enrichment (SELEX). Both purified CD70 protein and CD70-expressing cells were used to isolate target-specific aptamer. After certain rounds of protein-based SELEX and cell-SELEX (ten and seven rounds, respectively), Aptamer 928 (hereafter Apt928) with high affinity and specificity for CD70 was obtained. The specific binding of the aptamer to its target could block interaction of CD70 with CD27 (known as CD70 receptor). Dissociation constant of Apt928 was calculated to be 66 nmol L

Identifiants

DOI: 10.1016/j.talanta.2018.10.063 PMID: 30609550
pubmed: 30609550
pii: S0039-9140(18)31103-2
doi: 10.1016/j.talanta.2018.10.063
pii:
doi:

Substances chimiques

Aptamers, Nucleotide 0
CD27 Ligand 0
CD70 protein, human 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

399-405

Informations de copyright

Copyright © 2018 Elsevier B.V. All rights reserved.

Auteurs

Payam Bayat (P)

Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Seyed Mohammad Taghdisi (SM)

Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.

Houshang Rafatpanah (H)

Inflammation and Inflammatory Diseases Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Khalil Abnous (K)

Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

Mohammad Ramezani (M)

Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: Ramezanim@mums.ac.ir.

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Aptamères nucléotidiques › In vitro selection of CD70 binding aptamer and...
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence nucléotidique › In vitro selection of CD70 binding aptamer and...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › In vitro selection of CD70 binding aptamer and...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Facteurs de nécrose tumorale › Antigènes CD70 › In vitro selection of CD70 binding aptamer and...
questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › In vitro selection of CD70 binding aptamer and...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › In vitro selection of CD70 binding aptamer and...
questionsmedicales.fr › Maladies endocriniennes › Troubles gonadiques › Maladies ovariennes › Tumeurs de l'ovaire › In vitro selection of CD70 binding aptamer and...