Surface plasmon resonance aptasensor for detection of human activated protein C.
Activated protein C
Aptasensor
DNA aptamer
Surface plasmon resonance
Journal
Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R
Informations de publication
Date de publication:
01 Mar 2019
01 Mar 2019
Historique:
received:
03
08
2018
revised:
28
09
2018
accepted:
01
10
2018
entrez:
6
1
2019
pubmed:
6
1
2019
medline:
13
2
2019
Statut:
ppublish
Résumé
The aim of this study is a highly sensitive and selective label-free surface plasmon resonance (SPR) aptasensor preparation for the specific detection of human activated protein C (APC). In the first step, DNA aptamer was complexed with N-methacryloyl-L-cysteine (MAC) monomer. Then, cyanamide and 2-hydroxyethyl methacrylate solution was mixed with the DNA-Apt/MAC complex. Two different SPR sensors (Random-DNA and HEMA-MAC polymeric films) were also prepared by following the same experimental procedure. The characterization of SPR aptasensors was done by contact angle, atomic force microscopy, and ellipsometer analysis. Selectivity studies of SPR aptasensors were performed in the presence of bovine serum albumin, hemoglobin and myoglobin. Desorption studies were performed by using 0.025 M NaCl solution. The limit of detection (LOD) and limit of quantification (LOQ) values of DNA-Apt SPR aptasensor was determined as 1.5 ng/mL and 5.2 ng/mL.
Identifiants
pubmed: 30609568
pii: S0039-9140(18)31036-1
doi: 10.1016/j.talanta.2018.10.007
pii:
doi:
Substances chimiques
Aptamers, Nucleotide
0
Protein C
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
528-533Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.