Electrochemiluminescent biosensor with DNA link for selective detection of human IgG based on steric hindrance.
Biosensing Techniques
/ methods
Cadmium Compounds
/ chemistry
DNA
/ chemistry
Electrochemistry
Electrodes
Europium
/ chemistry
Gold
/ chemistry
Humans
Immunoglobulin G
/ analysis
Limit of Detection
Luminescent Measurements
Models, Molecular
Nucleic Acid Conformation
Nucleic Acid Hybridization
Quantum Dots
/ chemistry
Selenium Compounds
/ chemistry
Biosensor
Digoxin-linked signaling DNA
Electrochemiluminescence
Europium (II) doped CdSe quantum dots
Human IgG
Steric hindrance effects
Journal
Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R
Informations de publication
Date de publication:
01 Mar 2019
01 Mar 2019
Historique:
received:
18
06
2018
revised:
27
09
2018
accepted:
27
10
2018
entrez:
6
1
2019
pubmed:
6
1
2019
medline:
12
2
2019
Statut:
ppublish
Résumé
A highly selective DNA-based electrochemiluminescence (ECL) based biosensor is described for the detection of human IgG. It is exploiting the effect of steric hindrance that affects the strength of the ECL signal in the presence of IgG. Digoxin-linked signaling DNA was specifically bound to IgG, and this causes steric hindrance which limits the ability of DNA to hybridize with capturing DNA attached to a gold electrode. Europium (II) doped CdSe quantum dots were covalently linked to the DNA in order to generate the ECL signal. Using this steric hindrance hybridization method, the ECL signal of the biosensor were proportional to the concentration of IgG with a wide linear range and a 14 pM detection limit. Conceivably, the method can be expanded to the detection of a wide range of proteins for which homologous recognition elements are available.
Identifiants
pubmed: 30609601
pii: S0039-9140(18)31138-X
doi: 10.1016/j.talanta.2018.10.092
pii:
doi:
Substances chimiques
Cadmium Compounds
0
Immunoglobulin G
0
Selenium Compounds
0
Europium
444W947O8O
Gold
7440-57-5
DNA
9007-49-2
cadmium selenide
A7F646JC5C
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
745-751Informations de copyright
Copyright © 2018 Elsevier B.V. All rights reserved.