Isolation of Human Peripheral Blood T-Lymphocytes.

Blood Cells / cytology Cell Separation / methods Centrifugation, Density Gradient / methods Ficoll Humans Leukocytes, Mononuclear / cytology T-Lymphocytes / cytology
Density gradient Ficoll PBMC T-Lymphocytes

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2019
Historique:
entrez: 6 1 2019
pubmed: 6 1 2019
medline: 20 8 2019
Statut: ppublish

Résumé

Peripheral blood is the most common source of T-lymphocytes for in vitro culture. Here, we present a simple and standardized method for small- or large-scale isolation of viable T-lymphocytes and other mononuclear cells from fresh peripheral blood or buffy coat blood samples using the density gradient centrifugation. T-cells obtained using the protocol described here can be used for a variety of downstream analysis, including cellular, molecular, and functional assays.

Identifiants

DOI: 10.1007/978-1-4939-9036-8_2 PMID: 30610593
pubmed: 30610593
doi: 10.1007/978-1-4939-9036-8_2
doi:

Substances chimiques

Ficoll 25702-74-3

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Pagination

11-17

Auteurs

Atish Kizhakeyil (A)

Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore. ATISH001@e.ntu.edu.sg.

Seow Theng Ong (ST)

Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore.

Mobashar Hussain Urf Turabe Fazil (MHUT)

Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore.

Madhavi Latha Somaraju Chalasani (MLS)

Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore.
Autoimmunity and Inflammation Program, Hospital for Special Surgery, New York, NY, USA.

Praseetha Prasannan (P)

Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore.

Navin Kumar Verma (NK)

Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Singapore, Singapore.

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Classifications MeSH

questionsmedicales.fr › Systèmes sanguin et immunitaire › Sang › Cellules sanguines › Isolation of Human Peripheral Blood T-Lymphocytes.
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Séparation cellulaire › Isolation of Human Peripheral Blood T-Lymphocytes.
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Ultracentrifugation › Centrifugation en gradient de densité › Isolation of Human Peripheral Blood T-Lymphocytes.
questionsmedicales.fr › Glucides › Polyosides › Ficoll › Isolation of Human Peripheral Blood T-Lymphocytes.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Isolation of Human Peripheral Blood T-Lymphocytes.
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Isolation of Human Peripheral Blood T-Lymphocytes.
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Lymphocytes T › Isolation of Human Peripheral Blood T-Lymphocytes.