Correlative Light and Electron Microscopy of Autophagosomes.
Autophagosome
Electron tomography
Live-cell imaging
Phagophore
Serial sectioning
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
6
1
2019
pubmed:
6
1
2019
medline:
25
6
2019
Statut:
ppublish
Résumé
Live-cell imaging has been widely used to study autophagosome biogenesis and maturation. When combined with correlative electron microscopy, this approach can be extended to reveal ultrastructural details in three dimensions. The resolution of electron microscopy is needed when membrane contact sites and tubular connections between organelles are studied.
Identifiants
pubmed: 30610698
doi: 10.1007/978-1-4939-8873-0_12
doi:
Substances chimiques
MAP1LC3A protein, human
0
Microtubule-Associated Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM