Intestinal organoids: A new paradigm for engineering intestinal epithelium in vitro.
Decellularized small intestine
Host-microbial interaction
Induced pluripotent stem cells
Organoids
Organs-on-a-Chips
Tissue engineering
Journal
Biomaterials
ISSN: 1878-5905
Titre abrégé: Biomaterials
Pays: Netherlands
ID NLM: 8100316
Informations de publication
Date de publication:
02 2019
02 2019
Historique:
received:
30
08
2018
revised:
22
11
2018
accepted:
08
12
2018
pubmed:
7
1
2019
medline:
19
5
2020
entrez:
7
1
2019
Statut:
ppublish
Résumé
In recent years, the advent of intestinal organoid culture systems has revolutionized in vitro studies of the small intestine epithelium. Intestinal organoids are derived from self-organizing and self-renewing intestinal stem cells and closely recapitulate the native intestinal epithelium. They therefore represent a more physiologically-relevant in vitro model than conventional cell cultures for studying intestinal development, biology and pathophysiology. Moreover, they represent a promising and unprecedented new tool in the realm of regenerative and personalized medicine. In this review, we outline the current approaches to develop intestinal organoids and describe the strategies used to induce complexity, multicellularity and modularity in organoid culture systems; this knowledge will contribute to improved biomimicry of the organoid culture system. We focus on co-culture systems and explore the convergence of organoid technology and engineering principals. Finally, we describe applications of intestinal organoids in various fields.
Identifiants
pubmed: 30612006
pii: S0142-9612(18)30828-7
doi: 10.1016/j.biomaterials.2018.12.006
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
195-214Informations de copyright
Copyright © 2018 Elsevier Ltd. All rights reserved.