The Notch repressor complex in Drosophila: in vivo analysis of Hairless mutants using overexpression experiments.
Drosophila melanogaster
Notch antagonist Hairless
Notch repression
Notch signalling
Overexpression
Repressor complex
Suppressor of Hairless
Journal
Development genes and evolution
ISSN: 1432-041X
Titre abrégé: Dev Genes Evol
Pays: Germany
ID NLM: 9613264
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
14
09
2018
accepted:
17
12
2018
pubmed:
7
1
2019
medline:
8
5
2019
entrez:
7
1
2019
Statut:
ppublish
Résumé
During development of higher animals, the Notch signalling pathway governs cell type specification by mediating appropriate gene expression responses. In the absence of signalling, Notch target genes are silenced by repressor complexes. In the model organism Drosophila melanogaster, the repressor complex includes the transcription factor Suppressor of Hairless [Su(H)] and Hairless (H) plus general co-repressors. Recent crystal structure analysis of the Drosophila Notch repressor revealed details of the Su(H)-H complex. They were confirmed by mutational analyses of either protein; however, only Su(H) mutants have been further studied in vivo. Here, we analyse three H variants predicted to affect Su(H) binding. To this end, amino acid replacements Phenylalanine 237, Leucines 245 and 247, as well as Tryptophan 258 to Alanine were introduced into the H protein. A cell-based reporter assay indicates substantial loss of Su(H) binding to the respective mutant proteins H
Identifiants
pubmed: 30612166
doi: 10.1007/s00427-018-00624-2
pii: 10.1007/s00427-018-00624-2
doi:
Substances chimiques
Drosophila Proteins
0
Receptors, Notch
0
Transcription Factors
0
H protein, Drosophila
148349-66-0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
13-24Références
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